Shortening of the Induction Period of Allergic Asthma in Cynomolgus Monkeys by Ascaris suum and House Dust Mite

Autor: Youichi Mataki, Takashi Kuwabara, Katsumasa Iwashita, Hirokazu Kawasaki, Masatsugu Sawada, Mika In
Jazyk: angličtina
Rok vydání: 2008
Předmět:
Male
Time Factors
Administration
Oral
Immunoglobulin E
medicine.disease_cause
Bronchoconstrictor Agents
Allergen
Airway resistance
Anti-Asthmatic Agents
Sensitization
Ascaris suum
Cells
Cultured
Methacholine Chloride
Inhalation
biology
Helminth Proteins
T-Lymphocytes
Helper-Inducer
respiratory system
medicine.anatomical_structure
Molecular Medicine
Bronchial Hyperreactivity
Injections
Intraperitoneal
medicine.drug
Receptors
CCR4
Prednisolone
Injections
Intramuscular
Fluticasone propionate
Administration
Inhalation
medicine
Animals
Antigens
Dermatophagoides
Pharmacology
House dust mite
business.industry
Airway Resistance
lcsh:RM1-950
Allergens
Intradermal Tests
biology.organism_classification
Asthma
respiratory tract diseases
Androstadienes
Disease Models
Animal
Macaca fascicularis
lcsh:Therapeutics. Pharmacology
Immunology
biology.protein
Leukocytes
Mononuclear
Fluticasone
Methacholine
Interleukin-4
Interleukin-5
business
Zdroj: Journal of Pharmacological Sciences, Vol 106, Iss 1, Pp 92-99 (2008)
ISSN: 1347-8613
Popis: The development of non-human primate models of asthma requires a period of time (e.g., 0.5 –1 year). To develop the models in a short period, male cynomolgus monkeys were sensitized with dinitrophenyl-Ascaris suum (DNP-As) allergen by intraperitoneal and intramuscular injection and by intratracheal inhalation. All sensitized animals developed positive intradermal skin reaction to DNP-As. Sensitization elevated allergen-specific IgE levels in serum, the number of CCR4-positive T helper lymphocytes in peripheral blood, and IL-4 and IL-5 releases from phorbol 12-myristate 13-acetate- and ionomycin-stimulated peripheral blood. In addition, allergen challenge induced increases in lung resistance, airway inflammation, and hyperresponsiveness to inhaled methacholine. Next, animals were sensitized with house dust mite extracts (HDM) under the similar procedure. In these animals sensitized with DNP-As or HDM, inhaled fluticasone propionate and oral prednisolone inhibited the allergen-induced airway hyperresponsiveness. Taken together, monkey asthma models were successfully developed by sensitization with DNP-As or HDM under a short-term protocol (within 7 weeks). These models should be useful for the evaluation of anti-inflammatory drugs for asthma treatment. Keywords:: airway hyperresponsiveness, bronchoalveolar lavage, CCR4-positive cell, cytokine, methacholine
Databáze: OpenAIRE
Externí odkaz: