Recombinant human uncoupling protein-3 increases thermogenesis in yeast cells
| Autor: | W. Hinz, P. Gazzotti, S. Grüninger, Bernard Faller, Michele Chiesi |
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| Rok vydání: | 1999 |
| Předmět: |
Hot Temperature
Cellular respiration Recombinant Fusion Proteins Biophysics Saccharomyces cerevisiae Oxidative phosphorylation Mitochondrion Biology Biochemistry Ion Channels Mitochondrial Proteins Structural Biology Uncoupling protein Genetics medicine Humans Uncoupling Protein 3 Molecular Biology Cell growth Respiration Skeletal muscle Thermogenesis Cell Biology Yeast Thermogenin Mitochondria medicine.anatomical_structure Carrier Proteins Cell Division |
| Zdroj: | FEBS Letters. 448:57-61 |
| ISSN: | 0014-5793 |
| Popis: | The long form of human uncoupling protein-3 (hUCP3L) is highly homologous to thermogenin (UCP1), the uncoupling protein of brown fat mitochondria, but is expressed predominantly in skeletal muscle. Its putative role is to regulate the coupling efficiency of oxidative phosphorylation and thus thermogenesis in skeletal muscle, a major thermogenic tissue in higher mammals. To study the functional relevance of hUCP3L, the protein was expressed in yeast cells under the control of the galactose promoter. Expression of hUCP3L induced a series of phenotype changes in the yeast cells. The cellular growth and the mitochondrial membrane potential were both diminished. The portion of cellular respiration coupled to oxidative phosphorylation decreased from 57% to 11% (P |
| Databáze: | OpenAIRE |
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