Effects of batroxobin treatment on the survival of random skin flaps in rats
| Autor: | Peng-Fu Xu, Yu-Zhi Jin, Pei-Yang Hu, Jun-Wei Wang, Chao-Yang Qi, Miaojie Fang, Xin-Yi Chen, Dingsheng Lin |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A 0301 basic medicine medicine.medical_specialty medicine.medical_treatment Immunology Blood viscosity Urology Neovascularization Physiologic Haematoxylin Surgical Flaps Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Malondialdehyde medicine Animals Immunology and Allergy Saline Pharmacology Superoxide Dismutase business.industry Microcirculation Batroxobin Laser Doppler velocimetry medicine.disease Thrombosis Vascular endothelial growth factor 030104 developmental biology chemistry 030220 oncology & carcinogenesis business |
| Zdroj: | International Immunopharmacology. 72:235-242 |
| ISSN: | 1567-5769 |
| Popis: | Objective Batroxobin is a medicinal preparation extracted from the venom of the Fer-de-Lance snake, and is used to lower blood viscosity, promote blood fibrinogen decomposition, and inhibit thrombosis. This research is to investigate whether batroxobin can improve the survival of random skin flaps in a rat model. Materials and methods Dorsal McFarlane flaps were harvested from 36 rats divided into two groups. Experimental group: Batroxobin was administered via the tail vein once daily. Control group: The same amount of normal saline was injected instead. On day 2, superoxide dismutase (SOD) and malondialdehyde (MDA) levels were measured. On day 7, tissue slices were stained with haematoxylin and eosin. Expression of vascular endothelial growth factor (VEGF) was immunohistochemically evaluated. Microcirculatory flow was measured by laser Doppler flowmetry. Flap angiography, using the lead oxide-gelatin injection technique, was performed with the aid of a soft X-ray machine. Results The batroxobin group exhibited a greater mean flap survival area, a better microcirculatory flow, and higher-level expression of SOD and VEGF compared with the control group. However, the MDA level was significantly reduced. Conclusion Batroxobin effectively improved the survival of random skin flaps. |
| Databáze: | OpenAIRE |
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