Fresh and frozen-thawed human sperm bind in a similar pattern to the zona pellucida in the hemizona assay
| Autor: | Gedalia Paz, Yehuda Hiss, Haim Yavetz, Zvi T. Homonnai, Ronni Gamzu, Leah Yogev |
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| Rok vydání: | 1992 |
| Předmět: |
Cryopreservation
Male Sperm-Ovum Interactions endocrine system Hot Temperature urogenital system Obstetrics and Gynecology Motile sperm Semen Biology Sperm Spermatozoa In vitro Andrology medicine.anatomical_structure Reproductive Medicine medicine Humans Female Zona pellucida reproductive and urinary physiology Sperm motility Zona Pellucida |
| Zdroj: | Fertility and sterility. 58(6) |
| ISSN: | 0015-0282 |
| Popis: | This study evaluated the impact of sperm cryopreservation on sperm quality. The HZA was used to test the binding capacity of fresh as opposed to frozen-thawed sperm from 12 donors. Fresh and frozen-thawed sperm motility was 47% +/- 1.5% and 24% +/- 3.8% (mean +/- SE), respectively. However, the number of sperm cells attached to the hemizonae was 75 +/- 12.0 and 74 +/- 11.9, respectively. We conclude that cryopreservation results in a reduced number of motile sperm cells but does not adversely affect the ability of rescued sperm cells to bind to the ZP. The study also supports the use of frozen-thawed rather than fresh donor sperm for control in the HZA procedure. |
| Databáze: | OpenAIRE |
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