Functional Heterogeneity of Splenic T Lymphocyte Subpopulations I. Determination of Splenic Subpopulations by the Use of Mitogenic Probes
| Autor: | J. D. Waterfield, G. Möller, R. D Ekstedt |
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| Rok vydání: | 1977 |
| Předmět: |
Male
T-Lymphocytes Immunology Cell chemical and pharmacologic phenomena Lymphocyte Activation Mice Recovery period In vivo Lectins Concanavalin A medicine Animals Receptor Cells Cultured biology Lectin DNA General Medicine T lymphocyte Molecular biology In vitro medicine.anatomical_structure biology.protein Female Spleen |
| Zdroj: | Scandinavian Journal of Immunology. 6:615-623 |
| ISSN: | 1365-3083 0300-9475 |
| Popis: | The results reported here suggest that there exist two T-cell-mitogenic responsive populations in the spleen--one that is responsive to concanavalin A (Con A) and a second one that is responsive to Con A and phytohemagglutinin (PHA). Using an in vitro restimulation system, we have found that a primary in vivo activation with Con A effectively suppresses the subsequent restimulation response to PHA. However, in the reverse experiment, a primary in vivo PHA activation had little effect on the ability of cells to be restimulated with Con A. The suppression seen was due to a refractory recovery period displayed by all cells after previous activation and not to a nonspecific steric hindrance of mitogenic activation receptors due to the lectin remaining on the cell surfaces. |
| Databáze: | OpenAIRE |
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