The Impact of Partial Weak Staining in Normal Breast Epithelium on the Reliability of Immunohistochemistry Results in HercepTest-positive Breast Cancer
| Autor: | Chantal Farra, Faysal Fedda, Ghazi Zaatari, Grece Issa, Arafat Tfayli, Hanin Ashkar, Fouad Boulos, Ayman Tawil |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cancer Research Pathology medicine.medical_specialty Receptor ErbB-2 Breast Neoplasms HercepTest 03 medical and health sciences 0302 clinical medicine Breast cancer Gene duplication Biomarkers Tumor medicine Humans Breast skin and connective tissue diseases In Situ Hybridization Fluorescence medicine.diagnostic_test business.industry Gene Amplification Middle Aged Prognosis medicine.disease Immunohistochemistry Epithelium Staining 030104 developmental biology medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Female business Normal breast Follow-Up Studies Fluorescence in situ hybridization |
| Zdroj: | Clinical Breast Cancer. 19:340-344 |
| ISSN: | 1526-8209 |
| Popis: | Introduction Although normal epithelial cells do not show human epidermal growth factor receptor-2 (HER2) gene amplification and should lack membrane staining by HER2 immunohistochemistry (IHC), HER2 staining in benign breast epithelium is occasionally encountered. The significance of this occurrence has not yet been adequately studied, and its associated American Society of Clinical Oncology/College of American Pathologists recommendations are vague. Our objective is to assess the correlation between HER2 IHC 3+ breast cancer cases with normal epithelium staining (NES) and their corresponding fluorescence in situ hybridization (FISH) results, and to suggest recommendations for interpretation. Materials and Methods A total of 154 breast cancer cases with HER2 IHC 3+ were reviewed. NES, along with other clinicopathologic characteristics, were recorded. NES was scored as present or absent. All study cases were sent for FISH testing. All cases, and particularly those that showed false positivity for IHC (positive IHC, negative FISH) were examined for NES. Results Of the 154 cases, 146 cases were FISH-positive (94.8%) and 2 failed FISH testing (1.3%). Conversely, 22% (34/154) of the cases showed NES for HER2. Of these 34 cases, 23 (67%) were FISH-amplified, 9 (26%) were FISH not amplified, and 2 failed FISH testing. Notably, all of the false-positive (FISH-negative) breast cancer cases showed some degree of positivity in normal breast epithelium. Conclusions Our findings, though descriptive, show a very strong association between NES and false-positive HER2 IHC. This confirms the need to carefully evaluate IHC-positive breast cancers for NES, and to have a low threshold for confirmatory testing by FISH. |
| Databáze: | OpenAIRE |
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