Lipopolysaccharide and cyclic AMP regulation of CB2 cannabinoid receptor levels in rat brain and mouse RAW 264.7 macrophages
| Autor: | Sucharita Das, Duane Moore, Daniel S. Zahm, Andrew J. Lechner, Michael M. Ndengele, Somnath Mukhopadhyay, Allyn C. Howlett, Jenelle D. Jones, Evelyn A. Williams |
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| Rok vydání: | 2006 |
| Předmět: |
Lipopolysaccharides
medicine.medical_specialty Cannabinoid receptor Lipopolysaccharide Immunology Cell Line Receptor Cannabinoid CB2 Mice chemistry.chemical_compound Prosencephalon Internal medicine Cyclic AMP medicine Cannabinoid receptor type 2 Animals Immunology and Allergy Cycloheximide Protein kinase A Receptor Protein Synthesis Inhibitors Forskolin Microglia Kinase Macrophages Membrane Proteins Cyclic AMP-Dependent Protein Kinases Molecular biology Rats Up-Regulation Endocrinology medicine.anatomical_structure Neurology chemistry Neurology (clinical) |
| Zdroj: | Journal of Neuroimmunology. 181:82-92 |
| ISSN: | 0165-5728 |
| DOI: | 10.1016/j.jneuroim.2006.08.002 |
| Popis: | CB 2 cannabinoid receptors exist in immune cells including macrophages. Affinity-purified antibodies against the CB 2 receptor identified a 45 kDa protein in rat brain, human tonsil and rat and mouse microglia, but not mouse N18TG2 neuroblastoma cells. Intracerebroventricular lipopolysaccharide (LPS) increased immunoreactive CB 2 receptors in brain membranes detected by Western blot. LPS increased immunodetectable CB 2 receptors in cultured RAW 264.7 macrophages, and this was partially attenuated by cyclohexamide or the protein kinase A and C inhibitors H8 and bis-indolylmaleimide. Forskolin or dibutyryl cyclic AMP increased CB 2 receptor immunoreactivity, suggesting the involvement of the cyclic AMP–protein kinase A–Cyclic AMP response element pathway in the regulation of CB 2 receptor levels. |
| Databáze: | OpenAIRE |
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