Improved hypocrellin A production in Shiraia bambusicola by light-dark shift
| Autor: | Chun Xiao Sun, Jian Wen Wang, Yan Jun Ma |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Light Photoperiod 030106 microbiology Biophysics Real-Time Polymerase Chain Reaction Industrial Microbiology 03 medical and health sciences chemistry.chemical_compound Ascomycota Gene Expression Regulation Fungal Medicinal fungi Radiology Nuclear Medicine and imaging Food science Perylene Mycelium chemistry.chemical_classification Reactive oxygen species Radiation NADPH oxidase Phenol Radiological and Ultrasound Technology biology Cytochrome c peroxidase Quinones Bambusicola Shiraia bambusicola biology.organism_classification 030104 developmental biology chemistry Cell culture biology.protein Reactive Oxygen Species |
| Zdroj: | Journal of Photochemistry and Photobiology B: Biology. 182:100-107 |
| ISSN: | 1011-1344 |
| DOI: | 10.1016/j.jphotobiol.2018.04.004 |
| Popis: | Hypocrellin A (HA) is a major bioactive perylenequinone from the fruiting body of Shiraia bambusicola used for the treatment of skin diseases and developed as a photodynamic therapy (PDT) agent against cancers and viruses. The mycelial culture of S. bambusicola under dark is a biotechnological alternative for HA production but with low yield. In this study, light and dark conditions were investigated to develop effective elicitation on HA production in the cultures. Our results showed the constant light at 200 lx stimulated HA production without any growth retardation of mycelia. A light/dark shift (24: 24 h) not only increased HA content in mycelia by 65%, but stimulated HA release into the medium with the highest total HA production 181.67 mg/L on day 8, about 73% increase over the dark control. Moreover, light/dark shifting induced the formation of smaller and more compact fungal pellets, suggesting a new effective strategy for large-scale production of HA in mycelium cultures. The light/dark shift up-regulated the expression levels of two reactive oxygen species (ROS) related genes including superoxide-generating NADPH oxidase (Nox) and cytochrome c peroxidase (CCP), and induced the generation of ROS. With the treatment of vitamin C, we found that ROS was involved in the up-regulated expression of key biosynthetical genes for hypocrellins and improved HA production. These results provide a basis for understanding the influence of light/dark shift on fungal metabolism and the application of a novel strategy for enhancing HA production in submerged Shiraia cultures. |
| Databáze: | OpenAIRE |
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