Effective Treatment of Cutaneous Mold Infections by Antimicrobial Blue Light That Is Potentiated by Quinine
| Autor: | Clinton K. Murray, David C. Hooper, Leon G. Leanse, Ying Wang, Carolina dos Anjos, Tianhong Dai |
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| Rok vydání: | 2021 |
| Předmět: |
Fusarium
Porphyrins Light Aspergillus flavus Aspergillus fumigatus Microbiology Cell wall Mice Major Articles and Brief Reports hemic and lymphatic diseases medicine Animals Immunology and Allergy Skin Diseases Infectious neoplasms Quinine ABL biology Chemistry Spores Fungal biology.organism_classification Antimicrobial Anti-Bacterial Agents Infectious Diseases Intracellular medicine.drug |
| Zdroj: | J Infect Dis |
| ISSN: | 1537-6613 0022-1899 |
| Popis: | Background Cutaneous mold infections commonly result from an array of traumatic injuries that involve direct inoculation of contaminated soil into wounds. Here, we explored the use of antimicrobial blue light (aBL; 405 nm wavelength) and the combination of aBL with quinine hydrochloride (aBL + Q-HCL) for the treatment of cutaneous mold infections. Methods Efficacy of aBL and aBL + Q-HCL in killing clinically important pathogenic molds (Aspergillus fumigatus, Aspergillus flavus, and Fusarium oxyprorum) was investigated. Ultraperformance liquid chromatography identified and quantified endogenous porphyrins in the mold conidia. Finally, a mouse model of dermabrasion wound infected with a bioluminescent variant of A. fumigatus was developed to investigate the efficacy of aBL in treating cutaneous mold infections. Results We demonstrated that mold conidia are tolerant to aBL, but Q-HCL enhances efficacy. Transmission electron microscopy revealed intracellular damage by aBL. aBL + Q-HCL resulted in intracellular and cell wall damage. Porphyrins were observed in all mold strains, with A. fumigatus having the highest concentration. aBL and aBL + Q-HCL effectively reduced the burden of A. fumigatus within an established dermabrasion infection and limited recurrence posttreatment. Conclusions aBL and aBL + Q-HCL may offer a novel approach for the treatment of mold infections. |
| Databáze: | OpenAIRE |
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