Evaluation of the diagnostic potential of urinary N-Acetyltyramine-O,β-glucuronide (NATOG) as diagnostic biomarker for Onchocerca volvulus infection
| Autor: | Lieve Dillen, Steven Silber, Petra Vinken, Alexander Yaw Debrah, Ole Lagatie, Emmanuel Njumbe Ediage, Christ Nolten, Luc Diels, Lieven J. Stuyver, Linda Batsa Debrah |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Population Tyramine Urine Onchocerciasis Microfilaria Gastroenterology 03 medical and health sciences Ivermectin Glucuronides Tandem Mass Spectrometry Internal medicine Onchocerciasis Ocular parasitic diseases medicine Animals Humans Diagnostic education Microfilariae Lymphatic filariasis education.field_of_study biology Research NATOG Biomarker biology.organism_classification medicine.disease Onchocerca volvulus 030104 developmental biology Infectious Diseases River blindness Immunology Neglected tropical diseases Biomarker (medicine) Parasitology Female Biomarkers medicine.drug Chromatography Liquid |
| Zdroj: | Parasites & Vectors |
| ISSN: | 1756-3305 |
| Popis: | Background Onchocerciasis, also known as river blindness is one of the neglected tropical diseases affecting millions of people, mainly in sub-Saharan Africa and is caused by the filarial nematode Onchocerca volvulus. Efforts to eliminate this disease are ongoing and are based on mass drug administration programs with the microfilaricide ivermectin. In order to monitor the efficacy of these programs, there is an unmet need for diagnostic tools capable of identifying infected patients. We have investigated the diagnostic potential of urinary N-acetyltyramine-O,β-glucuronide (NATOG), which is a promising O. volvulus specific biomarker previously identified by urine metabolome analysis. Methods A liquid chromatography tandem mass spectrometry (LC-MS/MS) method was used to assess the stability characteristics of NATOG and to evaluate the levels of NATOG in study samples. An LC-fluorescence method was also developed. Results Stability characteristics of NATOG were investigated and shown to be ideally suited for use in tropical settings. Also, an easy and more accessible method based on liquid chromatography coupled to fluorescence detection was developed and shown to have the necessary sensitivity (limit of quantification 1 μM). Furthermore, we have evaluated the levels of NATOG in a population of 98 nodule-positive individuals from Ghana with no or low levels of microfilaria in the skin and compared them with the levels observed in different control groups (endemic controls (n = 50), non-endemic controls (n = 18) and lymphatic filariasis (n = 51). Only a few (5 %) of nodule-positive individuals showed an increased level (> 10 μM) of NATOG and there was no statistical difference between the nodule-positive individuals and the control groups (P > 0.05). Conclusions Results of the present study indicate the limited potential of NATOG as a diagnostic biomarker for O. volvulus infection in amicrofilaridermic individuals. Electronic supplementary material The online version of this article (doi:10.1186/s13071-016-1582-6) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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