Enrichment in spontaneous and complement dependent rosette forming cell populations using ficoll-hypaque gradient centrifugation
| Autor: | A. E. Bachmann, E. D. Carosella, R. W. Rohwedder, I. Paulone |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Pharmacology
B-Lymphocytes Chemistry T-Lymphocytes Cell Ficoll Cell Separation Cell Biology Gradient centrifugation E rosettes Molecular biology Peripheral blood Rosette (zoology) Cellular and Molecular Neuroscience medicine.anatomical_structure Immunology Centrifugation Density Gradient Immunologic Techniques medicine Humans Molecular Medicine Molecular Biology |
| Zdroj: | Experientia. 32:1484-1486 |
| ISSN: | 1420-9071 0014-4754 |
| DOI: | 10.1007/bf01937450 |
| Popis: | Peripheral blood human T (TL) and B (BL) lymphocytes were separated by continuous Ficoll-Hypaque (FH) gradients. BL were found at the 1050 density interfase (70.5% EAC rosettes) with no TL (0% EAC rosettes); while at the 1068 density interfase there was an enrichment of TL (68% E rosettes) with very few BL (8.5% EAC rosettes). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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