Structure-Based Approaches to Improving Selectivity through Utilizing Explicit Water Molecules: Discovery of Selective β-Secretase (BACE1) Inhibitors over BACE2
Autor: | Kazuki Fujimoto, Shigeru Ando, Yutaka Tonomura, Shuhei Yoshida, Harrie J.M. Gijsen, Shiho Yamamoto, Diederik Moechars, Naoki Kanegawa, Frederik J. R. Rombouts, Hisanori Ito, Kouki Fuchino, Shinji Suzuki, Takahiko Yamamoto, Eriko Matsuoka, Genta Tadano, Naoya Asada, Ken-ichi Kusakabe |
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Rok vydání: | 2021 |
Předmět: |
Propynyl
01 natural sciences Cocrystal 03 medical and health sciences chemistry.chemical_compound Structure-Activity Relationship Alzheimer Disease Drug Discovery Oxazines Molecule Aspartic Acid Endopeptidases Humans Enzyme Inhibitors 030304 developmental biology 0303 health sciences Chemistry Water Combinatorial chemistry 0104 chemical sciences 010404 medicinal & biomolecular chemistry Drug Design β secretase Molecular Medicine Verubecestat Structure based Amyloid Precursor Protein Secretases Selectivity High homology |
Zdroj: | Journal of medicinal chemistry. 64(6) |
ISSN: | 1520-4804 |
Popis: | BACE1 is an attractive target for disease-modifying treatment of Alzheimer's disease. BACE2, having high homology around the catalytic site, poses a critical challenge to identifying selective BACE1 inhibitors. Recent evidence indicated that BACE2 has various roles in peripheral tissues and the brain, and therefore, the chronic use of nonselective inhibitors may cause side effects derived from BACE2 inhibition. Crystallographic analysis of the nonselective inhibitor verubecestat identified explicit water molecules with different levels of free energy in the S2' pocket. Structure-based design targeting them enabled the identification of propynyl oxazine 3 with improved selectivity. Further optimization efforts led to the discovery of compound 6 with high selectivity. The cocrystal structures of 7, a close analogue of 6, bound to BACE1 and BACE2 confirmed that one of the explicit water molecules is displaced by the propynyl group, suggesting that the difference in the relative water displacement cost may contribute to the improved selectivity. |
Databáze: | OpenAIRE |
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