Developmental changes in the adhesive disk during Giardia differentiation
| Autor: | Malin E.-L. Weiland, Frances D. Gillin, Shanda R. Birkeland, Sarah E. Pacocha, Michael J. Cipriano, Jadwiga Winiecka-Krusnell, Andrew G. McArthur, Barbara J. Davids, Staffan G. Svärd, Daniel Palm, Ewert Linder |
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| Rok vydání: | 2005 |
| Předmět: |
Molecular Sequence Data
Protozoan Proteins Biology medicine.disease_cause Organelle Morphogenesis medicine Animals Giardia lamblia Parasite hosting Electrophoresis Gel Two-Dimensional Amino Acid Sequence RNA Messenger Serial analysis of gene expression Cytoskeleton Molecular Biology Peptide sequence Ventral disc Regulation of gene expression Sequence Homology Amino Acid Gene Expression Regulation Developmental Cell biology Gene Expression Regulation Parasitology Sequence Alignment RNA Protozoan |
| Zdroj: | Molecular and Biochemical Parasitology. 141:199-207 |
| ISSN: | 0166-6851 |
| Popis: | Giardia lamblia is a protozoan parasite infecting the upper mammalian small intestine. Infection relies upon the ability of the parasite to attach to the intestine via a unique cytoskeletal organelle, the ventral disk. We determined the composition and structure of the disk throughout the life cycle of the parasite and identified a new disk protein, SALP-1. SALP-1 is an immunodominant protein related to striated fiber-assemblin (SFA). The disk is disassembled during encystation and stored as four fragments in the immobile cyst. Serial Analysis of Gene Expression (SAGE) showed that the mRNA levels of the disk proteins decreased in encystation but two-dimensional protein gels showed that the protein levels were more constant. The parasite emerges without a functional disk but the four disk fragments are quickly reassembled into two new disks on the dividing, early excysting form. Thus, disk proteins are stored within the cyst, ready to be used in the rapid steps of excystation. |
| Databáze: | OpenAIRE |
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