Classical light scattering quantitation of protein aggregates: off-line spectroscopy versus HPLC detection
| Autor: | V. Levi, M. Kunitani, Gavin Dollinger, C. Apicella, S. Rana, S. Wolfe |
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| Rok vydání: | 1997 |
| Předmět: |
Quality Control
Clinical Biochemistry Analytical chemistry Pharmaceutical Science Protein aggregation Mass spectrometry Fluorescence spectroscopy Light scattering Analytical Chemistry Nephelometry and Turbidimetry Fluorometer Drug Discovery Calibration Humans Scattering Radiation Fluorometry Spectroscopy Chromatography High Pressure Liquid Serum Albumin Chromatography Chemistry Proteins Reproducibility of Results Molecular Weight Cuvette Models Chemical Chromatography Gel Toluene |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 16:573-586 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/s0731-7085(97)00191-x |
| Popis: | This paper describes the development and validation of a new off-line approach to quantitate both covalent and noncovalent, in-solution aggregates present in protein formulations and compares the new assay to established HPLC methods. This off-line analysis is well suited for use in QC release testing, formulation development and stability indicating applications. An inexpensive, continuous source HPLC fluorometer has been adapted with the addition of second order filters for use as a sensitive right-angle scatterometer which can determine the molecular weight of protein aggregates in solution. When used as an HPLC detector, right-angle light scattering is a sensitive method which can determine the molecular weight of peaks separable by HPLC, thus discriminating between monomers of different conformations and aggregates. The weight-averaged molecular weight of aggregate peaks can be calculated with system calibration, yielding the average number of monomers per aggregate. If the protein concentration is high enough for an adequate signal, the off-line technique of right-angle light scattering of protein formulations has advantages of convenience and speed over the HPLC approach. Samples are placed in standard fluorometer cuvettes and toluene is used as a calibrator. Data are presented which show the off-line (static) method to be extremely rapid, rugged and precise. The accuracy of this approach is demonstrated through cross-validation to traditional GPC analysis of protein aggregate distributions. This non-invasive light scattering approach is particularly useful when non-covalent protein aggregation is reversible and readily altered by chromatographic separations typically used for characterizing aggregates. |
| Databáze: | OpenAIRE |
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