The multi-xenobiotic resistance (MXR) efflux activity in hemocytes of Mytilus edulis is mediated by an ATP binding cassette transporter of class C (ABCC) principally inducible in eosinophilic granulocytes

Autor: Céline Boulangé-Lecomte, Damien Rioult, Matthieu Marin, Agnès Poret, Jennifer Pasquier, Christophe Minier, Frank Le Foll, Imane Abbas
Přispěvatelé: Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS), Stem Cell and Microenvironment Laboratory, Weill Cornell Medicine [Qatar], Laboratoire d'Ecotoxicologie - Milieux Aquatiques (LEMA), Normandie Université (NU)-Normandie Université (NU)
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Hemocytes
Mytilus edulis
Health
Toxicology and Mutagenesis
[SDE.MCG]Environmental Sciences/Global Changes
Population
ATP-binding cassette transporter
010501 environmental sciences
Aquatic Science
01 natural sciences
Flow cytometry
03 medical and health sciences
[SDV.EE.ECO]Life Sciences [q-bio]/Ecology
environment/Ecosystems
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]
medicine
Animals
ATP Binding Cassette Transporter
Subfamily B
Member 1
education
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
0105 earth and related environmental sciences
Fluorescent Dyes
[SDV.EE.SANT]Life Sciences [q-bio]/Ecology
environment/Health
0303 health sciences
education.field_of_study
Innate immune system
medicine.diagnostic_test
biology
Transporter
Biological Transport
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
Anatomy
biology.organism_classification
Flow Cytometry
Fluoresceins
Molecular biology
Mytilus
Hemocyte migration
ATP-Binding Cassette Transporters
Efflux
[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
Water Pollutants
Chemical
Granulocytes
Zdroj: Aquatic Toxicology
Aquatic Toxicology, Elsevier, 2014, 153, pp.98-109. ⟨10.1016/j.aquatox.2013.11.012⟩
ISSN: 0166-445X
DOI: 10.1016/j.aquatox.2013.11.012⟩
Popis: In marine and estuarine species, immunotoxic and/or immunomodulatory mechanisms are the crossroad of interactions between xenobiotics, microorganisms and physicochemical variations of the environment. In mussels, immunity relies exclusively on innate responses carried out by cells collectively called hemocytes and found in the open hemolymphatic circulatory system of these organisms. However, hemocytes do not form a homogenous population of immune cells since distinct subtypes of mussel blood cells can be distinguished by cytochemistry, flow cytometry or cell motility analysis. Previous studies have also shown that these cells are able to efflux xenobiotics by means of ATP binding cassette (ABC) transporter activities conferring a multixenobiotic resistance (MXR) phenotype. ABC transporters corresponding to vertebrate class B/P-glycoprotein (P-gp) and to class C/multidrug resistance related protein (MRP) are characterized in Mytilidae. Herein, we have investigated the relative contributions of ABCB- and ABCC-mediated efflux within the different hemocyte subpopulations of Mytilus edulis mussels, collected from areas differentially impacted by chemical contaminants in Normandy (France). RT-PCR analyses provide evidence for the presence of ABCB and ABCC transporters transcripts in hemocytes. Immunodetection of ABCB/P-gp with the monoclonal antibody UIC2 in living hemocytes revealed that expression was restricted to granular structures of spread cells. Efflux transporter activities, with calcein-AM as fluorescent probe, were measured by combining flow cytometry to accurate Coulter cell size measurements in order to get a cell-volume normalized fluorescence concentration. In these conditions, basal fluorescence levels were higher in hemocytes originating from Yport (control site) than in cells collected from the harbor of Le Havre, where mussels are more exposed to with persistent pollutants. By using specific ABCB/P-gp (verapamil, PSC833, zosuquidar) and ABCC/MRP (MK571) blockers, we show that MXR activity is only carried out by MRP-type transporters in M. edulis hemocytes. In addition, cell-type-gated flow cytometry and calculation of the MXR activity factor indicate that ABCC-efflux activity is higher and more inducible in eosinophilic granulocytes than in other hemocyte subtypes. We conclude that, in the hemocytes of M. edulis, MXR phenotype is mediated by an ABCC/MRP-type transporter activity principally supported by eosinophilic granulocytes. A role for ABC transporters in hemocyte migration is discussed.
Databáze: OpenAIRE
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