Direct-method-aided phasing of MAD data
| Autor: | Quan Hao, Yiwei Liu, Hai-fu Fan, Steven E. Ealick, Yunrong Gu |
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| Rok vydání: | 2001 |
| Předmět: |
Models
Molecular animal structures Anomalous scattering Protein Conformation Computer science business.industry Direct method Context (language use) General Medicine Crystallography X-Ray Phaser Optics Structural Biology Data Interpretation Statistical Direct methods Protein human Humans Scattering Radiation business Adenosine Kinase Algorithm |
| Zdroj: | Acta Crystallographica Section D Biological Crystallography. 57:250-253 |
| ISSN: | 0907-4449 |
| DOI: | 10.1107/s0907444900018606 |
| Popis: | The direct methods of breaking the phase ambiguity intrinsic in one-wavelength anomalous scattering (OAS) data and MAD phasing are powerful methods in their own rights. In a different context, in addition to their success in phasing OAS data, direct methods can also be useful in the treatment of MAD data. The idea has been tested with the MAD data at 2.5 A resolution from the protein human adenosine kinase [Mathews et al. (1998), Biochemistry, 37, 15607--15620]. The results showed that the incorporation of direct methods in MAD phasing led to a significant improvement of phases over those obtained from the conventional MAD phasing method alone, as indicated by improved map correlation coefficients (with the existing model), reduced phase errors by 4.5 degrees and improved map connectivity. |
| Databáze: | OpenAIRE |
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