The contribution of p53 and Y chromosome long arm genes to regulation of apoptosis in mouse testis
| Autor: | Tomasz Lech, Józefa Styrna, Katarzyna Kotarska |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Yq deletion Mice 129 Strain Genotype Congenic Apoptosis Reproductive technology Biology Y chromosome 03 medical and health sciences Mice Congenic Endocrinology Y Chromosome Testis Genetics medicine Animals Spermatogenesis Molecular Biology Gene Mice Knockout TUNEL assay male germ cells Sexual Development Age Factors Gene Expression Regulation Developmental Molecular biology spermatogenesis Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Phenotype Reproductive Medicine p53 knockout Animal Science and Zoology Tumor Suppressor Protein p53 Germ cell Developmental Biology Biotechnology |
| Popis: | Apoptosis of excessive or defective germ cells is a natural process occurring in mammalian testes. Tumour suppressor protein p53 is involved in this process both in developing and adult male gonads. Its contribution to testicular physiology is known to be modified by genetic background. The aim of this study was to evaluate the combined influence of the p53 and Y chromosome long arm genes on male germ cell apoptosis. Knockout of the transformation related protein 53 (Trp53) gene was introduced into congenic strains: B10.BR (intact Y chromosome) and B10.BR-Ydel (Y chromosome with a deletion in the long arm). The level of apoptosis in the testes of 19-day-old and 3-month-old male mice was determined using the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate in situ nick-end labelling (TUNEL) method. The study revealed that although p53 is involved in germ cell apoptosis in peripubertal testes, this process can also be mediated by p53-independent mechanisms. However, activation of p53-independent apoptotic pathways in the absence of the p53 protein requires engagement of the multicopy Yq genes and was not observed in gonads of B10.BR-Ydel-p53−/− males. The role of Yq genes in the regulation of testicular apoptosis seems to be restricted to the initial wave of spermatogenesis and is not evident in adult gonads. The study confirmed, instead, that p53 does participate in spontaneous apoptosis in mature testes. |
| Databáze: | OpenAIRE |
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