Development of a new screening method for the detection of antibiotic residues in muscle tissues using liquid chromatography and high resolution mass spectrometry with a LC-LTQ-Orbitrap instrument
| Autor: | Dominique Hurtaud-Pessel, E. Verdon, T. Jagadeshwar-Reddy |
|---|---|
| Přispěvatelé: | Unité de Toxicologie des Contaminants, Laboratoire de Fougères - ANSES, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Fougeres Laboratory, Unit veterinary drug residues, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) |
| Rok vydání: | 2011 |
| Předmět: |
medicine.drug_class
analysis muscle Health Toxicology and Mutagenesis Automated data processing Antibiotics High resolution 02 engineering and technology Toxicology Orbitrap Mass spectrometry 01 natural sciences Mass Spectrometry law.invention residues Liquid chromatography–mass spectrometry law [CHIM.ANAL]Chemical Sciences/Analytical chemistry antibiotic medicine Screening method liquid chromatography Animals tissues Muscle Skeletal LC-LTQ-orbitrap Chromatography Chemistry 010401 analytical chemistry Public Health Environmental and Occupational Health Life Sciences General Chemistry General Medicine 021001 nanoscience & nanotechnology Drug Residues 3. Good health 0104 chemical sciences Anti-Bacterial Agents Meat Products high resolution mass spectrometry 0210 nano-technology Food Science Chromatography Liquid |
| Zdroj: | Food Addit Contam Part A Chem Anal Control Expo Risk Assess Food Addit Contam Part A Chem Anal Control Expo Risk Assess, 2011, 28 (10), pp.1340-51. ⟨10.1080/19440049.2011.605772⟩ Food Additives and Contaminants Food Additives and Contaminants, 2011, 28 (10), pp.1340-1351. ⟨10.1080/19440049.2011.605772⟩ |
| ISSN: | 1944-0057 |
| DOI: | 10.1080/19440049.2011.605772⟩ |
| Popis: | International audience; In the present work, a liquid chromatography- high resolution mass spectrometry method was developed for the screening in meat of a wide range of antibiotics used in veterinary medicine. Full scan mode under high resolution mass spectral conditions using LTQ-Orbitrap mass spectrometer with resolving power 60.000 FWHM was applied for analysis of the samples. Samples were prepared using two extractions protocols prior to LC-MS analysis. The scope of the method focuses on the screening of the following main families of antibacterial veterinary drugs: penicillins, cephalosporins, sulphonamides, macrolides, tetracyclines, aminoglucosides and quinolones. Compounds were successfully identified in spiked samples by their accurate mass and LC retention times from the acquired full-scan chromatogram. An automatic process of the data using ToxId software allows a rapid treatment of the data. Analyses of muscle tissues from real samples collected on antibiotic treated animals have been carried out using the above methodology and antibiotic residues have been identified unambiguously. Further analysis of the data of the real samples allowed the identification of the targeted antibiotic residues but also the non targeted compounds, such as some of their metabolites. |
| Databáze: | OpenAIRE |
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