An extracellular Leptospira interrogans leucine-rich repeat protein binds human E- and VE-cadherins
| Autor: | Jenifer Coburn, Patrick England, Jacques Bellalou, Mathieu Picardeau, Hiromi Sato, Robert A. Gaultney, Azad Eshghi, Tara J. Moriarty, Sébastien Brûlé, Isabelle Miras, Ahmed Haouz |
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| Přispěvatelé: | Biologie des Spirochètes / Biology of Spirochetes, Institut Pasteur [Paris], Genome British Columbia Proteomics Centre [Victoria] (UVic-Genome BC Proteomics Centre), University of Victoria [Canada] (UVIC), Biophysique Moléculaire (Plate-forme), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Cristallographie (Plateforme) - Crystallography (Platform), Medical College of Wisconsin, Production de Protéines Recombinantes (Plate-Forme) (PRPF), University of Toronto (University of Toronto), National Institute of Allergy and InfectiousDiseases, Grant/Award Number:R01AI112920, NIH, Grant/Award Number:R01AI112920, Pasteur Foundation, BPIFrance, Grant/Award Number: FUI 14, French Ministry of Research, Grant/Award Number:ANR‐08‐MIE-018, ANR-08-MIEN-0018,LEPTOVIR,Les leptospires : de la génétique à la pathogénèse(2008), Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2018 |
| Předmět: |
MESH: Adhesins
Bacterial MESH: Leptospira interrogans [SDV]Life Sciences [q-bio] Leucine-Rich Repeat Proteins Genome MESH: Cadherins MESH: Cricetulus MESH: Animals MESH: Proteins adherence MESH: Antigens CD Leptospira 0303 health sciences leucine-rich biology MESH: Leptospirosis spirochaete Cadherins 3. Good health [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Leptospira interrogans Immunology Guinea Pigs CHO Cells Leucine-rich repeat Microbiology Article MESH: Guinea Pigs Cell Line 03 medical and health sciences Cricetulus MESH: CHO Cells Antigens CD Virology Animals Humans Leptospirosis Adhesins Bacterial Gene 030304 developmental biology MESH: Humans 030306 microbiology Binding protein Proteins biology.organism_classification infection MESH: Cell Line Bacterial adhesin cadherin Bacteria |
| Zdroj: | Cell Microbiol Cellular Microbiology Cellular Microbiology, Wiley, 2019, 21 (2), pp.e12949. ⟨10.1111/cmi.12949⟩ Cellular Microbiology, 2019, 21 (2), pp.e12949. ⟨10.1111/cmi.12949⟩ |
| ISSN: | 1462-5822 1462-5814 |
| Popis: | International audience; Pathogenic Leptospira bacteria are the causative agents of leptospirosis, a zoonotic disease affecting animals and humans worldwide. These pathogenic species have the ability to rapidly cross host tissue barriers by a yet unknown mechanism. A comparative analysis of pathogens and saprophytes revealed a higher abundance of genes encoding proteins with leucine-rich repeat (LRR) domains in the genomes of pathogens. In other bacterial pathogens, proteins with LRR domains have been shown to be involved in mediating host cell attachment and invasion. One protein from the pathogenic species Leptospira interrogans, LIC10831, has been previously analysed via X-ray crystallography, with findings suggesting it may be an important bacterial adhesin. Herein we show that LIC10831 elicits an antibody response in infected animals, is actively secreted by the bacterium, and binds human E- and VE-cadherins. These results provide biochemical and cellular evidences of LRR protein-mediated host-pathogen interactions and identify a new multireceptor binding protein from this infectious Leptospira species. |
| Databáze: | OpenAIRE |
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