N-Acyl Dopamines Induce Apoptosis in Endometrial Stromal Cells from Patients with Endometriosis
| Autor: | Irina A. Fedorova-Gogolina, Alina M. Gamisonia, Chupalav M. Eldarov, M. G. Akimov, Mikhail Bobrov, Marina N. Yushina, Vladimir V. Bezuglov, Gennady T. Sukhikh, S. V. Pavlovich, Natalia Gretskaya |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
endometriosis
Programmed cell death CB1 receptor Cannabinoid receptor Stromal cell QH301-705.5 Endometriosis selective toxicity Catalysis Article Inorganic Chemistry medicine Cytotoxic T cell Biology (General) Physical and Theoretical Chemistry endocannabinoid system QD1-999 Molecular Biology Spectroscopy reactive oxygen species Chemistry Organic Chemistry apoptosis General Medicine medicine.disease Endocannabinoid system Computer Science Applications Apoptosis Cancer research Ovarian Endometriosis endometrial stromal cells N-acyl dopamines |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 19 International Journal of Molecular Sciences, Vol 22, Iss 10648, p 10648 (2021) |
| ISSN: | 1422-0067 |
| Popis: | Endometriosis is characterized by the formation and development of endometrial tissues outside the uterus, based on an imbalance between proliferation and cell death, leading to the uncontrolled growth of ectopic foci. The potential target for the regulation of these processes is the endocannabinoid system, which was found to be involved in the migration, proliferation, and survival of tumor cells. In this paper, we investigated the effect of endocannabinoid-like compounds from the N-acyl dopamine (NADA) family on the viability of stromal cells from ectopic and eutopic endometrium of patients with ovarian endometriosis. N-arachidonoyldopamine, N-docosahexaenoyldopamine, and N-oleoyldopamine have been shown to have a five-times-more-selective cytotoxic effect on endometrioid stromal cells. To study the mechanisms of the toxic effect, inhibitory analysis, measurements of caspase-3/9 activity, reactive oxygen species, and the mitochondrial membrane potential were performed. It was found that NADA induced apoptosis via an intrinsic pathway through the CB1 receptor and downstream serine palmitoyltransferase, NO synthase activation, increased ROS production, and mitochondrial dysfunction. The higher selectivity of NADA for endometriotic stromal cells and the current lack of effective drug treatment can be considered positive factors for further research of these compounds as possible therapeutic agents against endometriosis. |
| Databáze: | OpenAIRE |
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