Berberine Antifungal Activity in Fluconazole-Resistant Pathogenic Yeasts: Action Mechanism Evaluated by Flow Cytometry and Biofilm Growth Inhibition in Candida spp
| Autor: | Thalles B. Grangeiro, Daniel Domingues Freitas, Manoel Odorico de Moraes, Bruno C. Cavalcanti, Rosana de Sousa Campos, Francisca Bruna Stefany Aires do Nascimento, João Batista de Andrade Neto, Rose Anny Costa Silva, Larissa Nara Dantas de Andrade, Anderson Ramos da Silva, Cecília Rocha da Silva, Hemerson Iury Ferreira Magalhães, Letícia Serpa Sampaio, Hélio Vitoriano Nobre Júnior |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Antifungal Agents Berberine Berberina Berberis aristata Microbial Sensitivity Tests Cell Line Microbiology Mice 03 medical and health sciences chemistry.chemical_compound L Cells Drug Resistance Fungal medicine Animals Humans Pharmacology (medical) DNA Fungal Mycological Typing Techniques Fluconazole Mechanisms of Action: Physiological Effects Candida Cell Proliferation Pharmacology Cryptococcus neoformans biology Broth microdilution Candidiasis Biofilm Fungal genetics Cryptococcosis biology.organism_classification Molecular Typing 030104 developmental biology Infectious Diseases chemistry Fluconazol Biofilms Mitochondrial Membranes Phellodendron amurense Fungos medicine.drug |
| Zdroj: | Antimicrobial Agents and Chemotherapy Repositório Institucional da Universidade Federal do Ceará (UFC) Universidade Federal do Ceará (UFC) instacron:UFC |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.01846-15 |
| Popis: | The incidence of fungal infections and, in particular, the incidence of fungal antibiotic resistance, which is associated with biofilm formation, have significantly increased, contributing to morbidity and mortality. Thus, new therapeutic strategies need to be developed. In this context, natural products have emerged as a major source of possible antifungal agents. Berberine is a protoberberine-type isoquinoline alkaloid isolated from the roots, rhizomes, and stem bark of natural herbs, such as Berberis aquifolium , Berberis vulgaris , Berberis aristata , and Hydrastis canadensis , and of Phellodendron amurense . Berberine has been proven to have broad antibacterial and antifungal activity. In the present study, the potential antifungal effect of berberine against fluconazole-resistant Candida and Cryptococcus neoformans strains, as well as against the biofilm form of Candida spp., was assessed. The antifungal effect of berberine was determined by a broth microdilution method (the M27-A3 method of the Clinical and Laboratory Standards Institute) and flow cytometry techniques, in which the probable mechanism of action of the compound was also assessed. For biofilm assessment, a colorimetric 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was used to determine the susceptibility of sessile cells. The isolates used in the study belonged to the Laboratory of Bioprospection and Experiments in Yeast (LABEL) of the Federal University of Ceará. After 24 and 72 h, fluconazole-resistant Candida and Cryptococcus neoformans strains showed berberine MICs equal to 8 μg/ml and 16 μg/ml, respectively. Cytometric analysis showed that treatment with berberine caused alterations to the integrity of the plasma and mitochondrial membranes and DNA damage, which led to cell death, probably by apoptosis. Assessment of biofilm-forming isolates after treatment showed statistically significant reductions in biofilm cell activity ( P < 0.001). |
| Databáze: | OpenAIRE |
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