Polymerase chain reaction in detection of CMV DNA in renal allograft recipients
| Autor: | L. C. Williams, P. B. Doak, K. F. Powell, H. Antoszewska, Stephen R. Munn, W. T. Lee, M. C. Croxson, D. Verran, J. Collins |
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| Rok vydání: | 1992 |
| Předmět: |
Adult
Male medicine.medical_treatment Cytomegalovirus medicine.disease_cause Antibodies Viral Asymptomatic Polymerase Chain Reaction Herpesviridae Serology Betaherpesvirinae Internal Medicine Leukocytes Medicine Humans biology business.industry Viral culture virus diseases Immunosuppression Middle Aged biology.organism_classification Virology Kidney Transplantation Immunology Cytomegalovirus Infections DNA Viral Female Virus Activation Viral disease medicine.symptom business Biomarkers |
| Zdroj: | Australian and New Zealand journal of medicine. 22(3) |
| ISSN: | 0004-8291 |
| Popis: | This study investigates the w of polymerase chain reaction (PCR) in comparison with viral culture and serology for monitoring of cytomegalovirus (CMV) infection in 21 consecutive renal allograft recipients mated with a quadruple immunosuppression protocol. In addition, an attempt is made to explore the significance of quantitation of CMV signals obtained from peripheral blood leucocytes. CMV infection developed in 16 patients with seven of the patients having organ involve mat. All of these 16 patients bad a fourfold rise in antibody as well as positive identification of CMV DNA in peripheral blood leucocytes by PCR. Blood viral cultures were negative in two of these patients. All five patients who remained PCR negative also remained culture negative with no antibody change. PCR detected CMV infection on average 15 days and 20 days earlier than viral culture and serology respectively. All except one of the patients with CMV organ involvement had an initial peak of CMV DNA followed by prolonged carriage of detectable CMV. The majority of patients with fever only or asymptomatic CMV infection had a transient peak of CMV DNA. A high incidence of CMV disease with organ involvement occurred in seronegative recipients of kidneys from seropositive donors (3/5) and in seropositive recipients Of kidneys from seronegative donors (3/7). OKT3 was associated with a higher incidence of CMV organ involvement compared to Antilymphocytic globulin (3/5 v 4/16) but there was a higher incidence of CMV mismatched patients in the OKT3 created group. This study confirmed the high incidence of CMV infection in renal allograft recipients on an aggressive immunosuppression regimen. The detection of CMV DNA in peripheral blood leucocytes by PCR is a sensitive and specific marker of CMV infection. It enables an earlier diagnosis of CMV infection. The precise role for monitoring of CMV DNA levels is yet to be fully defined. |
| Databáze: | OpenAIRE |
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