High-Dimensional Single-Cell Cartography Reveals Novel Skeletal Muscle-Resident Cell Populations
| Autor: | Gary J. He, Aurélien Corneau, Tom H. Cheung, Elisa Negroni, Hiroshi Sakai, M. Mona Siu, Raymond Wan, Shahragim Tajbakhsh, Lorenzo Giordani, Justin Y. C. Law, Fabien Le Grand |
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| Přispěvatelé: | Centre de recherche en Myologie – U974 SU-INSERM, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Hong Kong University of Science and Technology (HKUST), Cellules Souches et Développement / Stem Cells and Development, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Max Planck Institute for Heart and Lung Research (MPI-HLR), Max-Planck-Gesellschaft, Plateforme Cytométrie Pitié-Salpêtrière (LUMIC-CYPS), Unité Mixte de Service d'Imagerie et de Cytométrie [CHU Saint-Antoine] (UMS LUMIC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), This study was supported by ANR/RGC (Agence Nationale pour la Recherche/Research Grant Council) grant ANR-14-CE11-0026/A-HKUST604/14 (to F.L.G. and T.C.), an Association Française contre les Myopathies/AFM Telethon grant to F.L.G., the Hong Kong Research Grant Council (research grants C6015-14G, C6003-14G, C6009-15G, AoE/M-604/16, and T13-607/12R to T.H.C.), and the Croucher Innovation Award (T.H.C.)., We thank Catherine Blanc (CyPS Facility) for technical support. We thank Bruno Cadot, Christophe Combadière, Glenda Comai, and Vincent Mouly for providing transgenic mice. We thank Gilian Butler-Browne, Florian Bentzinger, David Sassoon, Rémi Mounier, and Capucine Trollet for commenting on the manuscript., Centre de Recherche en Myologie, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Unité Mixte de Service d'Imagerie et de Cytométrie [CHU Saint-Antoine] (UMS LUMIC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU) |
| Rok vydání: | 2018 |
| Předmět: |
MESH: Muscle Fibers
Skeletal/metabolism Cell type MESH: Muscle Fibers Skeletal/cytology Satellite Cells Skeletal Muscle [SDV]Life Sciences [q-bio] Cell Muscle Fibers Skeletal skeletal muscle stem cells Biology Muscle Development Myoblasts 03 medical and health sciences Mice 0302 clinical medicine MESH: Stem Cells/metabolism medicine Compartment (development) MESH: Myoblasts/metabolism MESH: Satellite Cells Skeletal Muscle/metabolism Animals MESH: Animals Cell Lineage MESH: Satellite Cells Skeletal Muscle/cytology MESH: Cell Lineage/genetics Molecular Biology MESH: Mice 030304 developmental biology satellite cells 0303 health sciences single-cell RNA-seq Regeneration (biology) MESH: Cell Differentiation/genetics Stem Cells Mesenchymal stem cell Scleraxis MESH: Myoblasts/cytology Skeletal muscle Cell Differentiation Cell Biology Cell biology Transplantation MESH: Stem Cells/cytology medicine.anatomical_structure MESH: Muscle Development/genetics CyTOF Single-Cell Analysis 030217 neurology & neurosurgery MESH: Single-Cell Analysis |
| Zdroj: | Molecular Cell Molecular Cell, Elsevier, 2019, 74 (3), pp.609-621.e6. ⟨10.1016/j.molcel.2019.02.026⟩ Molecular Cell, 2019, 74 (3), pp.609-621.e6. ⟨10.1016/j.molcel.2019.02.026⟩ |
| ISSN: | 1097-4164 1097-2765 |
| DOI: | 10.1016/j.molcel.2019.02.026⟩ |
| Popis: | International audience; Adult tissue repair and regeneration require stem-progenitor cells that can self-renew and generate differentiated progeny. Skeletal muscle regenerative capacity relies on muscle satellite cells (MuSCs) and their interplay with different cell types within the niche. However, our understanding of skeletal muscle tissue cellular composition is limited. Here, using a combined approach of single-cell RNA sequencing and mass cytometry, we precisely mapped 10 different mononuclear cell types in adult mouse muscle. We also characterized gene signatures and determined key discriminating markers of each cell type. We identified two previously understudied cell populations in the interstitial compartment. One expresses the transcription factor scleraxis and generated tenocytes in vitro. The second expresses markers of smooth muscle and mesenchymal cells (SMMCs) and, while distinct from MuSCs, exhibited myogenic potential and promoted MuSC engraftment following transplantation. The blueprint presented here yields crucial insights into muscle-resident cell-type identities and can be exploited to study muscle diseases. |
| Databáze: | OpenAIRE |
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