Candida auris Direct Detection from Surveillance Swabs, Blood, and Urine Using a Laboratory-Developed PCR Method
| Autor: | Jean E. Barth, Paula Snippes Vagnone, Senait A Herring, Seanne P. Buckwalter, Jenna Rasmusson, Nancy L. Wengenack, Katrina M Henn, Katelyn M Johnson, Katelyn A. Reed, Jill L Fischer, Lory K. Steinmetz, Robert C. Walchak, Jolene M. Koelsch, Priya Sampathkumar, Nicole M Zinsmaster |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Candida auris Specific detection Pcr assay Plant Science Urine yeast Rapid detection Melting curve analysis Article 03 medical and health sciences Medicine lcsh:QH301-705.5 Ecology Evolution Behavior and Systematics 030304 developmental biology 0303 health sciences 030306 microbiology business.industry Fungal pathogen Virology PCR lcsh:Biology (General) surveillance identification Pcr method business |
| Zdroj: | Journal of Fungi Volume 6 Issue 4 Journal of Fungi, Vol 6, Iss 224, p 224 (2020) |
| ISSN: | 2309-608X |
| Popis: | Candida auris is an emerging fungal pathogen with cases reported in countries around the world and in 19 states within the United States as of August 2020. The CDC has recommended that hospitals perform active surveillance upon admission for patients with the appropriate risk factors. Currently, active surveillance requires that local hospitals send surveillance swabs to a public health laboratory for analysis. In this work, a real-time PCR assay was developed for the specific detection of C. auris from surveillance swabs, blood, and urine to enable rapid detection of this pathogen. The assay uses commercially available primers and reporter probes and it was verified on the LightCycler 480 PCR platform. Contrived specimens and prospectively collected composite groin/axilla surveillance swabs were used to validate the assay. The performance of the PCR assay on surveillance swabs was also compared to a second PCR assay targeting C. auris that was performed at the Minnesota Department of Health&ndash Public Health Laboratory (MDH-PHL). Our PCR assay is able to detect and differentiate C. auris from closely related Candida species such as C. duobushaemulonii, C. haemulonii, and C. pseudohaemulonii on the basis of melting curve temperature differences. |
| Databáze: | OpenAIRE |
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