| Autor: |
R.W. Rorie, T.D. Lester, D.W. Gliedt, R.W. McNew, G.F. Miller |
| Rok vydání: |
1994 |
| Předmět: |
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| Zdroj: |
Theriogenology. 42:385-395 |
| ISSN: |
0093-691X |
| Popis: |
Experiment 1 compared the development of 2- to 4-cell bovine embryos cultured in synthetic oviductal fluid with 20% fetal calf serum or 3.2% BSA and in the presence of oviductal cells, cumulus cells, or medium alone. More embryos developed in medium with serum, regardless of culture method (P=0.063). Oviductal cell co-culture resulted in more embryos developing to at least the morula stage (Por=0.066). The number of blastocysts was increased by the use of serum instead of BSA in cumulus cell co-culture medium (P0.001). Regardless of culture method, a similar percentage of embryos were excellent or good quality when BSA was used in medium (Por=0.400). Addition of serum to oviductal cell co-culture medium increased the number of excellent or good quality embryos (P=0.019). Experiment 2 further compared the development of 2-cell or 3- to 4-cell embryos co-cultured with oviductal cell suspensions in serum-supplemented synthetic oviductal fluid or M-199 medium. More 3- to 4-cell than 2-cell embryos developed to at least the morula stage (P0.001). More embryos developed to at least the morula stage in synthetic oviductal fluid (P=0.083). Neither initial embryo cell stage nor medium type influenced the percentage of developing embryos that achieved the blastocyst stage or final morphological quality of embryos (Por=0.535). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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