Response of macrophages in rat skeletal muscle after eccentric exercise

Autor: Xinkai Yu, Shu-Chen Wang, Qun Zuo, Wei-Wei Chao
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
medicine.medical_specialty
mannose receptor C type 2
(Mrc2)
arginase-1
(Arg-1)
Antigens
Differentiation
Myelomonocytic
Receptors
Cell Surface
Eccentric exercise
CCL2
Rats
Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
Antigens
CD
Internal medicine
Physical Conditioning
Animal
medicine
inducible NO synthase
(iNOS)
Macrophage
Regeneration
Animals
Orthopedics and Sports Medicine
Muscle injury
Muscle
Skeletal
CC chemokine ligand 2
(Ccl2)
lcsh:R5-920
Messenger RNA
Macrophage phenotype
myoglobin
(Mb)
business.industry
CD68
Myoglobin
Regeneration (biology)
Macrophages
Skeletal muscle
Phenotype
Rats
030104 developmental biology
Endocrinology
medicine.anatomical_structure
Surgery
Original Article
cyclooxygenase-2
(COX-2)
lcsh:Medicine (General)
business
CD163
030217 neurology & neurosurgery
C-type lectin domain family 10 member
(Clec10a)
Zdroj: Chinese Journal of Traumatology
Chinese Journal of Traumatology, Vol 21, Iss 2, Pp 88-95 (2018)
ISSN: 1008-1275
Popis: Purpose: Macrophages are known to be important for healing numerous injured tissues depending on their functional phenotypes in response to different stimuli. The objective of this study was to reveal macrophage phenotypic changes involved in exercise-induced skeletal muscle injury and regeneration. Methods: Adult male Sprague-Dawley rats experienced one session of downhill running (16° decline, 16 m/min) for 90 min. After exercise the blood and soleus muscles were collected at 0 h, 6 h, 12 h, 1 d, 2 d, 3 d, 1 w and 2 w after exercise, separately. Results: It was showed that CD68+ M1 macrophages mainly infiltrated into muscle necrotic sites at 1–3 d, while CD163+ M2 macrophages were present in muscles from 0 h to 2 weeks after exercise. Using transmission electron microscopy, we observed activated satellite cells 1 d after exercise. Th1-associated transcripts of iNOS and Ccl2 were inhibited post exercise, while COX-2 mRNA was dramatically increased 12 h after running (p
Databáze: OpenAIRE
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