Contractile protein system in the asexual stages of the malaria parasitePlasmodium falciparum
| Autor: | Anton R. Dluzewski, L. H. Bannister, Graham H. Mitchell, Catherina O'Shaughnessy, Walter Gratzer, Sarah E. Webb, Ruth E. Fowler, Jennifer C. Pinder |
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| Rok vydání: | 1996 |
| Předmět: |
Phalloidin
medicine.drug_class Molecular Sequence Data Plasmodium falciparum Protozoan Proteins Antibodies Protozoan macromolecular substances Myosins Biology Immunofluorescence Monoclonal antibody chemistry.chemical_compound Myosin medicine Animals Humans Amino Acid Sequence Peptide sequence Antiserum medicine.diagnostic_test Antibodies Monoclonal biology.organism_classification Molecular biology Actins Infectious Diseases Microscopy Fluorescence chemistry Polyclonal antibodies biology.protein Animal Science and Zoology Parasitology |
| Zdroj: | Parasitology. 112:451-457 |
| ISSN: | 1469-8161 0031-1820 |
| Popis: | SUMMARYF-actin was detected in asexual-stagePlasmodium falciparumparasites by fluorescence microscopy of blood films stained with fluorescent phalloidin derivatives. F-actin was present at all stages of development and appeared diffusely distributed in trophic parasites, but merozoites stained strongly at the poles and peripheries. No filament bundles could be discerned. A similar distribution was obtained by immunofluorescence with 2 polyclonal anti-actin antibodies, one of which was directed against a peptide sequence present only in parasite actin (as inferred from the DNA sequence of the gene). A monoclonal anti-actin antibody stained very mature or rupturing schizonts but not immature parasites. Myosin was identified in immunoblots of parasite protein extracts by several monoclonal anti-skeletal muscle myosin antibodies, as well as by a polyclonal antiserum directed against a consensus conserved myosin sequence (IQ motif). The identity of the polypeptides recognized by these antibodies was confirmed by overlaying blots with biotinylated F-actin. The antiserum and one of the monoclonal antibodies were used in immunofluorescence studies and were found to stain all blood-stage parasites, with maximal intensity towards the poles of merozoites. Our results are consistent with the presence of an actomyosin motor system in the blood-stage malaria parasite. |
| Databáze: | OpenAIRE |
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