Accu-Beads as a quality control measure for manual and automated methods of measuring sperm concentration—an observational study
| Autor: | Darian D. Vernon, William R. Boone, H. Lee Higdon, Angela M. Houwing, Jane E. Johnson |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Quality Control Accuracy and precision Computer science media_common.quotation_subject Cell Separation Andrology Control measure Genetics Humans Quality (business) Genetics (clinical) media_common Automation Laboratory Sperm Count urogenital system Reproducibility of Results Obstetrics and Gynecology General Medicine Sperm Microspheres Reproductive Medicine Technological Innovation Observational study Developmental Biology Biomedical engineering |
| Zdroj: | Journal of Assisted Reproduction and Genetics. 31:25-33 |
| ISSN: | 1573-7330 1058-0468 |
| Popis: | To determine the precision and accuracy of Accu-Beads and their utility as a quality control product for manual and automated measurements of sperm concentration.This observational study was performed at an Assisted Reproductive Technology laboratory in a tertiary-care, university hospital. To simulate sperm concentration, bead concentrations were measured with the use of a manual and an automated method.The manual counts did not vary significantly from the automated counts regardless of the concentration. However, the counts did vary between lots of low concentration of Accu-Beads and between the two different types of fixed counting chambers. The two bead concentrations used in this study were below the 95 % confidence interval for the values listed by the manufacturer.In our laboratory, Accu-Beads met enough of the requirements of a good control material to be acceptable for daily quality control use, especially if we set our own ranges of acceptability for each vial of Accu-Beads. It is necessary to evaluate each new lot of Accu-Beads when they are received and again if they are used with a different counting chamber. |
| Databáze: | OpenAIRE |
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