Diagnosis of Avian bornavirus infection in psittaciformes by serum antibody detection and reverse transcription polymerase chain reaction assay using feather calami
| Autor: | Anelle Kerski, Arne H. de Kloet, Siwo R. de Kloet |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Molecular Sequence Data
Enzyme-Linked Immunosorbent Assay Biology Antibodies Viral Psittaciformes Serology Parrots Proventricular dilatation disease Animals Base Sequence General Veterinary Bird Diseases Reverse Transcriptase Polymerase Chain Reaction Mononegavirales Infections Feathers Virology Nucleoprotein Reverse transcription polymerase chain reaction Bornaviridae Feather visual_art visual_art.visual_art_medium biology.protein RNA Viral Sample collection Antibody |
| Zdroj: | Journal of Veterinary Diagnostic Investigation. 23:421-429 |
| ISSN: | 1943-4936 1040-6387 |
| DOI: | 10.1177/1040638711403406 |
| Popis: | Avian bornavirus (ABV) is the causative agent of proventricular dilatation disease (PDD), a highly devastating and contagious disease of psittacines (parrots and parakeets), which has resulted in the death of many captive birds. Accurate diagnosis of bornavirus infection is therefore important for the identification and isolation of infected birds. The current study showed that nonvascular contour (chest) feather calami provide a ready and minimally invasive source of RNA for the detection of ABV by reverse transcription polymerase chain reaction (RT-PCR). Storage of the feathers at room temperature for at least a month did not affect the results. Serological analysis by enzyme-linked immunosorbent assay (ELISA) showed that identification of anti-bornaviral nucleoprotein P40 antibodies can identify many birds with a past or present infection. The presence of anti-avian bornaviral P24 phosphoprotein and P16 matrix protein antibodies was quite variable, rendering these antibodies less useful for diagnosis of ABV infection. The significance of the present findings is that the use of nonvascular feathers as a source of RNA allows sample collection under conditions where storage of other samples would be difficult. Serum detection by ELISA of anti-P40 antibodies allows the identification of infected birds when RT-PCR fails. |
| Databáze: | OpenAIRE |
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