Novel insights into plasma biomarker candidates in patients with chronic mountain sickness based on proteomics

Autor: Linhua Ji, Peili Zhang, Sen Cui, Chuanchuan Liu, Zhanquan Li, Zhancui Dang, Faman Yang, Yingzhong Yang, Shengyan Wang, Jie Liu, Ri-Li Ge, Huihui Liu, Jie Ma
Rok vydání: 2021
Předmět:
Male
Proteomics
0301 basic medicine
Altitude Sickness
Pharmacology
Biochemistry
Pathogenesis
0302 clinical medicine
Tandem Mass Spectrometry
Ethnicity
Protein Interaction Maps
Phosphoglycerate kinase 1
Diagnostics & Biomarkers
Research Articles
health care economics and organizations
chronic mountain sickness
chemistry.chemical_classification
education.field_of_study
thioredoxin-1
hemoglobin beta chain
Middle Aged
Chronic mountain sickness
Biomarker (medicine)
Adult
China
Bioinformatics
Biophysics
Omics
Oxidative phosphorylation
Biology
03 medical and health sciences
medicine
Humans
education
Molecular Biology
Reactive oxygen species
Sequence Analysis
RNA
biomarkers
Hydrogen peroxide catabolic process
Cell Biology
medicine.disease
Oxidative Stress
Gene Ontology
030104 developmental biology
chemistry
Chronic Disease
Reactive Oxygen Species
phosphoglycerate kinase 1
030217 neurology & neurosurgery
Chromatography
Liquid
Zdroj: Bioscience Reports
ISSN: 1573-4935
0144-8463
DOI: 10.1042/bsr20202219
Popis: Chronic mountain sickness (CMS) is a progressive incapacitating syndrome induced by lifelong exposure to hypoxia. In the present study, proteomic analysis was used to identify the differentially expressed proteins (DEPs) and then evaluate the potential plasma biomarkers between CMS and non-CMS groups. A total of 145 DEPs were detected in CMS Han Chinese people who live in the plateau (CMS-HPu), among which 89 were significantly up-regulated and 56 were significantly down-regulated. GO enrichment analysis showed that various biological processes were enriched, including the hydrogen peroxide metabolic/catabolic process, reactive oxygen species (ROS) metabolic, and acute inflammatory response. Protein–protein interaction analysis showed that antioxidant activity, the hydrogen peroxide catabolic process and peroxidase activity were primarily mapped in interaction proteins. Nine modules showed significantly clustering based on WGCNA analysis, with two being the most significant, and GO analysis showed that proteins of both modules were primarily enriched in oxidative stress-related biological processes. Four DEPs increased in CMS patients were evaluated as the candidate biomarkers, and three showed significant AUC: hemoglobin β chain (HB-β), thioredoxin-1 (TRX1), and phosphoglycerate kinase 1 (PGK1). The present study provides insights into the pathogenesis of CMS and further evaluates the potentially biomarkers for its prevention and treatment of it.
Databáze: OpenAIRE
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