Histopathological study of ovaries of Rhipicephalus sanguineus (Acari: Ixodidae) exposed to different thymol concentrations
| Autor: | Renata da Silva Matos, Bruno Rodrigues Sampieri, Maria Izabel Camargo-Mathias, Tatiane Pinheiro Lopes Novato, Rafael Neodini Remedio, Karim Christina Scopinho Furquim, Erik Daemon, Laryssa Xavier Araújo |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Veterinary medicine Pathology medicine.medical_specialty Rhipicephalus sanguineus Oviposition chemistry.chemical_compound Dogs medicine Animals Humans Acari Thymol Acaricides General Veterinary biology Dose-Response Relationship Drug Plant Extracts Ovary General Medicine biology.organism_classification Infectious Diseases chemistry Insect Science Oocytes Parasitology Arachnid Vectors Female Rabbits Ixodidae |
| Zdroj: | Parasitology research. 113(12) |
| ISSN: | 1432-1955 |
| Popis: | Thymol is a monoterpene with proven acaricide action for several tick species. In addition to killing these ectoparasites, thymol can also reduce oviposition and egg hatch rate. However, the effects of thymol on the morphophysiology of tick ovaries are still unknown. Thus, the aim of this study was to evaluate the morphophysiological changes caused by this active principle in ovaries of Rhipicephalus sanguineus after a 6-day feeding period, through the application of morphohistochemical techniques. After the feeding period, a total of 50 females were divided into five groups and immersed in the following solutions: (I) distilled water (control), (II) 30% ethanol (control), (III) 1.25 mg/mL thymol, (IV) 2.5 mg/mL thymol, and (V) 5.0 mg/mL thymol. The experimental groups were kept in a climatic chamber (27 ± 1 °C; RH 80 ± 10%) for 5 days. After this period, morphological (hematoxylin/eosin) and histochemical (von Kossa) techniques were applied after remotion of the ovaries. The morphological results revealed large vacuoles in germ cells at different developmental stages and invaginations that represent deformations in the chorionic membrane. From the results obtained in this study, it was concluded that thymol interfered with the development of oocytes, which showed degeneration signs. The treatment containing 5.0 mg/mL thymol affected more accentuately the morphological development. Moreover, thymol also altered the calcium content of yolk granules, which generally showed an intense staining for this element. |
| Databáze: | OpenAIRE |
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