Synthesis and biological evaluation of some novel 1,2,3-triazole hybrids of myrrhanone B isolated from Commiphora mukul gum resin: Identification of potent antiproliferative leads active against prostate cancer cells (PC-3)
| Autor: | Shailaja Karri, Ramakrishna Sistla, Mallavadhani Venkata Uppuluri, Rajendra Sangaraju, Chandrashekhar Madasu |
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| Rok vydání: | 2020 |
| Předmět: |
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2 3-Triazole medicine.drug_class Population Anti-Inflammatory Agents Antineoplastic Agents 01 natural sciences Anti-inflammatory Structure-Activity Relationship 03 medical and health sciences chemistry.chemical_compound DU145 Cell Line Tumor Drug Discovery medicine Humans Glycoside Hydrolase Inhibitors education Commiphora IC50 030304 developmental biology Pharmacology 0303 health sciences education.field_of_study Molecular Structure 010405 organic chemistry Organic Chemistry General Medicine Triazoles Molecular biology Triterpenes Deoxyuridine 0104 chemical sciences G2 Phase Cell Cycle Checkpoints chemistry Apoptosis Cell culture Drug Screening Assays Antitumor Resins Plant |
| Zdroj: | European Journal of Medicinal Chemistry. 188:111974 |
| ISSN: | 0223-5234 |
| DOI: | 10.1016/j.ejmech.2019.111974 |
| Popis: | A series of 28 novel 1,2,3-triazole hybrids of myrrhanone B have been designed and synthesized by employing regioselective Cu catalyzed Huisgen 1,3-dipolar cycloaddition reaction in highly efficient manner. All the synthesized analogues were assessed for their antiproliferative potential against A549 (Lung), DU145 (Prostate), MDA-MB-231 (Breast), SiHa (Cervical), U87MG (Glioblastoma), PC-3 (Prostate), HT-29 (Colon), L132 (Normal lung) cell lines. Further, the synthesized hybrids have also been screened for anti-inflammatory activity (TNF-α and IL-1β) and α-glucosidase inhibitory activity. The biological results revealed that compound 11 (meta hydroxy phenyl 1,2,3–triazole) and compound 29 (deoxyuridine 1,2,3–triazole) found to be the most potent antiproliferative ones against PC-3 cell line. Compound 11 (IC50: 6.57 ± 0.62 μM) showed six folds more potent than parent compound 1 (IC50: 40.67 ± 2.2 μM) and displayed almost identical inhibitory activity with standard doxorubicin (IC50: 5.05 ± 0.25 μM), whereas compound 29 (IC50: 10.85 ± 0.90 μM) exhibited four folds more potent than parent myrrhanone B (1). In view of potent activity of compounds 11 and 29 they have been subjected to detailed flowcytometry analysis. Compound 29 treated cells significantly increased the SubG1 population of cells indicative of apoptosis compared to compound 11. Further, the results of anti-inflammatory studies indicated that compounds 3, 6, 9, 27, 28, 29 and 30 exhibited significant inhibitory activity against both TNF-α and IL-1β than the parent compound 1. Interestingly, compound 27 exhibited good activity towards inflammatory cytokines TNF-α (IC50: 7.83 ± 0.95 μM). Interestingly, α-glucosidase inhibitory assay results revealed that compounds 14 (IC50: 2.77 ± 0.59 μM) and 16 (IC50: 4.12 ± 0.77 μM) as the most potent ones. In fact, compound 14 exhibited highest activity and found to be several times more potent than the parent compound 1 as well as standard acarbose (IC50: 2124 ± 170 μM). |
| Databáze: | OpenAIRE |
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