Bovine Papillomavirus Type 13 Expression in the Urothelial Bladder Tumours of Cattle
| Autor: | Franco Roperto, Federica Corrado, Manuela Martano, Sante Roperto, Valeria Russo, Leonardo Leonardi, Marita Georgia Riccardi |
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| Přispěvatelé: | Roperto, Sante, Russo, Valeria, Leonardi, Leonardo, Martano, Manuela, Corrado, Federica, Riccardi, Marita Georgia, Roperto, FRANCO PEPPINO |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
040301 veterinary sciences viruses Cattle Diseases Fluorescent Antibody Technique urothelial tumours Immunofluorescence Polymerase Chain Reaction Virus 0403 veterinary science 03 medical and health sciences medicine Animals Bovine papillomavirus type 13 Deltapapillomavirus cattle urinary bladder Papillomaviridae Bovine papillomavirus 1 Bovine papillomavirus Urinary bladder Base Sequence General Veterinary General Immunology and Microbiology biology medicine.diagnostic_test Papillomavirus Infections 04 agricultural and veterinary sciences General Medicine biochemical phenomena metabolism and nutrition Amplicon biology.organism_classification Immunohistochemistry Virology Molecular biology 030104 developmental biology medicine.anatomical_structure Urinary Bladder Neoplasms GenBank DNA Viral Female Brazil |
| Popis: | Bovine papillomavirus type 13 (BPV-13), a novel Deltapapillomavirus, has been found associated with urothelial tumours of the urinary bladder of cattle grazing on lands infested with bracken fern. BPV-13 was detected in 28 of 39 urothelial tumours. Diagnosis was based on sequencing of L1 and E5 amplicons from tumour samples. The nucleotide sequences generated from these amplicons showed a 100% homology with the sequences of BPV-13 L1 and E5 DNA found in Brazil from a fibropapilloma of the ear in a cow and from equine sarcoids in two horses. GenBank accession number of our representative BPV-13 sequences is JQ798171.1. Furthermore, mRNA encoding BPV-13 E5 oncoprotein was also documented, and its expression was also shown by immunohistochemistry and immunofluorescence in the basal and suprabasal urothelial tumour cells. In twenty-three tumours, BPV-13 was simultaneously found with BPV-2, a Deltapapillomavirus genus, species 4. The latter virus was detected by amplifying and sequencing a 154-bp-sized DNA fragment of BPV-2 E5. In addition, BPV-13 by itself was seen to be expressed in five BPV-2-negative urothelial tumours. This study shows that BPV-13 is present in urothelial tumour cells thus sharing biological properties with BPV-1 and BPV-2. Although further studies are needed, BPV-13 appears to be another worldwide infectious agent responsible for a distressing disease causing severe economic losses in cattle industry. |
| Databáze: | OpenAIRE |
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