Genetic characterization of complex inter-recombinant HIV-1 strains circulating in Spain and reliability of distinct rapid subtyping tools
| Autor: | María José Pena, Eva Lospitao, Jorge del Romero, Vincent Soriano, Cristina Martín, Marisa López, Eva Ramírez de Arellano, África Holguín |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Male Genotype Anti-HIV Agents HIV Infections Genome Viral Biology medicine.disease_cause Polymerase Chain Reaction Genome Genetic recombination Virus law.invention law Virology Drug Resistance Viral medicine Humans Gene Phylogeny Recombination Genetic Genetics Mutation Phylogenetic tree Genetic Variation Reproducibility of Results virus diseases Subtyping CD4 Lymphocyte Count Infectious Diseases Spain HIV-1 Recombinant DNA Female |
| Zdroj: | Journal of Medical Virology. 80:383-391 |
| ISSN: | 1096-9071 0146-6615 |
| DOI: | 10.1002/jmv.21105 |
| Popis: | Genetic recombination and high rate of mutation increase HIV-1 diversity, allowing viruses to escape more easily from the host immune response or antiretroviral drugs. The recombinant nature of full-length HIV-1 genomic sequences derived from viruses infecting five epidemiologically unlinked individuals carrying HIV-1 non-B variants was investigated. Overlapping PCR amplifications followed by direct sequencing of viral products derived from plasma and phylogenetic analyses were carried out. Four viral sequences clustered with CRF06_cpx and one with CRF02_AG. However, subtyping of separate genes within the same genome revealed that four were recombinant forms involving different subtypes and/or CRFs with distinct breakpoints. Two specimens included CRF02_AG and CRF06_cpx sequences with several fragments from other HIV-1 clades along their genomes. Three rapid subtyping tools (Stanford, NCBI, and REGA) showed discrepant results when interpreting these viral sequences. This is the first description of CRF02_AG/CRF06_ cpx recombinants in Spain. The results highlight the tremendous heterogeneity of HIV-1 recombinant strains currently in circulation. |
| Databáze: | OpenAIRE |
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