A functional pectin methylesterase inhibitor protein (SolyPMEI) is expressed during tomato fruit ripening and interacts with PME-1

Autor: Rossana D'Avino, Daniela Bellincampi, Ida Barbara Reca, Thierry Giardina, Felice Cervone, Laura Camardella, Vincenzo Lionetti
Rok vydání: 2012
Předmět:
0106 biological sciences
Pectin
Arabidopsis
Plant Science
01 natural sciences
Pichia
Solanum lycopersicum
Enzyme Inhibitors
Phylogeny
Plant Proteins
2. Zero hunger
Actinidia deliciosa
0303 health sciences
food and beverages
Ripening
General Medicine
Inhibitor protein
Plants
Genetically Modified
Recombinant Proteins
cell-wall metabolism
Biochemistry
Pectins
Functional genomics
pectin methylesterase inhibitor
food.ingredient
DNA
Plant
Molecular Sequence Data
fruit ripening
Biology
Genes
Plant
Pectin methylesterification Pectin methylesterase inhibitor Fruit ripening Solanum lycopersicum
Cell wall
03 medical and health sciences
pectin methylesterification
Transformation
Genetic
food
Affinity chromatography
Tobacco
Genetics
Amino Acid Sequence
030304 developmental biology
solanum lycopersicum
Base Sequence
Esterification
Sequence Homology
Amino Acid
biology.organism_classification
Transformation (genetics)
Fruit
Carboxylic Ester Hydrolases
Agronomy and Crop Science
010606 plant biology & botany
Zdroj: Plant molecular biology 79 (2012): 429–442. doi:10.1007/s11103-012-9921-2
info:cnr-pdr/source/autori:Ida Barbara Reca (1) (4) Vincenzo Lionetti (1) Laura Camardella (2) Rossana D'Avino (2) Thierry Giardina (3) Felice Cervone (1) Daniela Bellincampi (1)/titolo:A functional pectin methylesterase inhibitor protein (SolyPMEI) is expressed during tomato fruit ripening and interacts with PME-1/doi:10.1007%2Fs11103-012-9921-2/rivista:Plant molecular biology/anno:2012/pagina_da:429/pagina_a:442/intervallo_pagine:429–442/volume:79
ISSN: 1573-5028
0167-4412
DOI: 10.1007/s11103-012-9921-2
Popis: A pectin methylesterase inhibitor (SolyPMEI) from tomato has been identified and characterised by a functional genomics approach. SolyPMEI is a cell wall protein sharing high similarity with Actinidia deliciosa PMEI (AdPMEI), the best characterised inhibitor from kiwi. It typically affects the activity of plant pectin methylesterases (PMEs) and is inactive against a microbial PME. SolyPMEI transcripts were mainly expressed in flower, pollen and ripe fruit where the protein accumulated at breaker and turning stages of ripening. The expression of SolyPMEI correlated during ripening with that of PME-1, the major fruit specific PME isoform. The interaction of SolyPMEI with PME-1 was demonstrated in ripe fruit by gel filtration and by immunoaffinity chromatography. The analysis of the zonal distribution of PME activity and the co-localization of SolyPMEI with high esterified pectins suggest that SolyPMEI regulates the spatial patterning of distribution of esterified pectins in fruit.
Databáze: OpenAIRE
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