Pollen morphology and variability of Tulipa hungarica Borb
| Autor: | Ćalić, D, Devrnja, N, Milojević, J, Zdravković-Korać, S, Tubić, L, Djuričković, MS, Vinterhalter, B |
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| Rok vydání: | 2012 |
| Předmět: |
0106 biological sciences
Fluorescein diacetate Morphology (linguistics) food and beverages 15. Life on land Biology medicine.disease_cause 010603 evolutionary biology 01 natural sciences Applied Microbiology and Biotechnology Staining Microspore size pollen grain pollen viability staining intensity Tulipa Microspore Pollen Botany otorhinolaryngologic diseases Genetics medicine Agronomy and Crop Science Molecular Biology 010606 plant biology & botany Biotechnology |
| Zdroj: | African Journal of Biotechnology; Vol 11, No 3 (2012); 616-620 |
| ISSN: | 1684-5315 |
| DOI: | 10.5897/ajb11.2404 |
| Popis: | Pollen morphology of the endemic species Tulipa hungarica Borb was investigated with the goal of diversity protection and conservation. Morphology of pollen was evaluated using a scanning electron microscopy. Pollen grain was monosulcate with perforate striate exine surface. Also, pollen of T. hungarica showed differences in size, shape, and staining intensity after aceto-orceine treatment. The smallest aceto-orcein treated microspore had a diameter of 13.65 μm, while the largest had a diameter of 60.15 μm. 4,6-Diamidino-2-phenylindole (DAPI) staining showed that fifty percent of investigated microspores had one nucleus, and the other half had two nuclei. A rapid method with fluorescein diacetate – FDA was used to determine pollen viability. Up to (80%) of analyzed microspores showed high viability.Key words: Microspore size, pollen grain, pollen viability, staining intensity, Tulipa. |
| Databáze: | OpenAIRE |
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