Glutamine synthetase modulation in the brain of rats subjected to deprivation of paradoxical sleep
| Autor: | M. Didier-Bazes, V. Roudier, M. Sallanon-Moulin, Monique Touret, Marcienne Tardy, C. Fages, Michel Jouvet |
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| Rok vydání: | 1994 |
| Předmět: |
Male
medicine.medical_specialty Gs alpha subunit Rapid eye movement sleep Sleep REM Cellular and Molecular Neuroscience chemistry.chemical_compound Glutamate-Ammonia Ligase Corticosterone Parietal Lobe Internal medicine Glutamine synthetase Glial Fibrillary Acidic Protein medicine Animals RNA Messenger Molecular Biology Cerebral Cortex Glial fibrillary acidic protein biology Brain Rats Inbred Strains Frontal Lobe Rats Cortex (botany) medicine.anatomical_structure Endocrinology nervous system chemistry Cerebral cortex biology.protein Sleep Deprivation Locus coeruleus |
| Zdroj: | ResearcherID |
| ISSN: | 0169-328X |
| DOI: | 10.1016/0169-328x(94)90038-8 |
| Popis: | Changes in the level of glutamine synthetase (GS), an enzyme mainly located in astrocytes, were investigated in rat brain after deprivation of paradoxical sleep (PSD) and during recovery. An immunotitration method was used to evaluate the relative level of GS in brain tissue. At the end of a 24 h PSD, a significant increase in GS protein was observed both in the frontoparietal cortex (CX) and in the locus coeruleus area (LC). Four hours later during recovery, the level of GS protein returned to normal level in the CX but fell below control levels in the LC. In contrast, in the CX, the level of glial fibrillary acidic protein, an astroglial marker, did not change after PSD or during recovery. GS mRNA was quantified in the entire cortex by northern blot hybridization using of an oligonucleotidic GS-cDNA probe. We observed an increase in the GS mRNA level in the cortex of PSD rats of the same magnitude as the increase in GS protein. Both GS mRNA and GS protein tended to return to control values 4 h later during recovery. These results are discussed with particular attention to stress effects and possible physiological mechanisms regarding the regulation of amino acid levels by neurotransmitters during prolonged waking or neuronal excitation. |
| Databáze: | OpenAIRE |
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