The Non-Essential Mycolic Acid Biosynthesis Genes hadA and hadC Contribute to the Physiology and Fitness of Mycobacterium smegmatis
| Autor: | Kaymeuang Cam, Antonio Peixoto, Françoise Laval, Joana F. da Silva Domingues, Anne Lemassu, Nathalie Eynard, Pauline Texier, Stevie Jamet, Nawel Slama, Annaïk Quémard, Mamadou Daffé |
|---|---|
| Přispěvatelé: | Institut de pharmacologie et de biologie structurale (IPBS), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Université Jean Moulin - Lyon 3 (UJML), Université de Lyon, Tuberculose et biologie des infections [IPBS, Toulouse], Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
Cell Survival Mutant Mycobacterium smegmatis Physiology lcsh:Medicine Mycobacterium Infections Nontuberculous Microbial Sensitivity Tests Microbiology Mycobacterium tuberculosis chemistry.chemical_compound Biosynthesis Bacterial Proteins [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology lcsh:Science Gene Multidisciplinary biology lcsh:R Biofilm biology.organism_classification Phenotype [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology chemistry Mycolic Acids lcsh:Q Bacterial outer membrane Research Article |
| Zdroj: | PLoS ONE PLoS ONE, 2015, 10 (12), pp.e0145883. ⟨10.1371/journal.pone.0145883⟩ PLoS ONE, Vol 10, Iss 12, p e0145883 (2015) |
| ISSN: | 1932-6203 |
| Popis: | Gram positive mycobacteria with a high GC content, such as the etiological agent of tuberculosis Mycobacterium tuberculosis, possess an outer membrane mainly composed of mycolic acids (MAs), the so-called mycomembrane, which is essential for the cell. About thirty genes are involved in the biosynthesis of MAs, which include the hadA, hadB and hadC genes that encode the dehydratases Fatty Acid Synthase type II (FAS-II) known to function as the heterodimers HadA-HadB and HadB-HadC. The present study shows that M. smegmatis cells remain viable in the absence of either HadA and HadC or both. Inactivation of HadC has a dramatic effect on the physiology and fitness of the mutant strains whereas that of HadA exacerbates the phenotype of a hadC deletion. The hadC mutants exhibit a novel MA profile, display a distinct colony morphology, are less aggregated, are impaired for sliding motility and biofilm development and are more resistant to detergent. Conversely, the hadC mutants are significantly more susceptible to low- and high-temperature and to selective toxic compounds, including several current anti-tubercular drugs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|