Correlation of Y-chromosome multiple segmental deletions and chromosomal anomalies in non-obstructive azoospermic males from northeastern China
| Autor: | J. Y. Lee, J. L. Jin, S. B. Li, G. N. Niu, R. L. Dai, Ruixue Wang, R. Z. Liu |
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| Rok vydání: | 2012 |
| Předmět: |
Genetics
Adult Male China Comparative Genomic Hybridization Chromosomes Human Y Pcr assay Sex Chromosome Disorders of Sex Development General Medicine Biology Middle Aged Y chromosome Correlation Young Adult Chromosome analysis Karyotyping Humans Chromosome Deletion Molecular Biology Infertility Male Sex Chromosome Aberrations Azoospermia Sequence Tagged Sites |
| Zdroj: | Genetics and molecular research : GMR. 11(3) |
| ISSN: | 1676-5680 |
| Popis: | We investigated the frequency and types of Y-chromosome microdeletions and chromosomal anomalies in non-obstructive azoospermic and severely oligozoospermic infertile males in northeastern China. The sample consisted of 519 infertile males (456 azoospermic, 63 severely oligozoospermic). PCR assays for Y-chromosome microdeletions and chromosome analysis were performed on all patients and controls. Array-comparative genomic hybridization was performed for three patients with chromosomal anomalies. Fifty-nine of 519 patients (11.37%) had Y-chromosome microdeletions. Microdeletions were found in 11.18% (51/456) of the non-obstructive azoospermic patients and in 12.7% (8/63) of the severely oligozoospermic patients. Eleven of 51 non-obstructive azoospermic patients with Y-chromosome microdeletions had multiple segmental deletions in the AZFb+c regions; four of these patients had chromosomal anomalies. Our sample from northeastern China had a higher frequency of microdeletions among severely oligozoospermic than among non-obstructive azoospermic males. |
| Databáze: | OpenAIRE |
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