Antitumor Effect of the Ethanolic Extract from Seeds of Euphorbia lathyris in Colorectal Cancer
| Autor: | Rosario Martínez, Gloria Perazzoli, Milagros Galisteo, Laura Cabeza, Jesús M. Porres, Raúl Ortiz, Cristina Mesas, Consolación Melguizo, Jose Prados, María López-Jurado |
|---|---|
| Přispěvatelé: | [Mesas,C, Ortíz,R, Cabeza,L, Perazzoli,G, Melguizo,C, Prados,J] Institute of Biopathology and Regenerative Medicine (IBIMER), Center of Biomedical Research (CIBM), University of Granada, Granada, Spain. [Mesas,C, Prados,J] Department of Anatomy and Embryology, Faculty of Medicine, University of Granada, Granada, Spain. [Mesas,C, Prados,J] Instituto Biosanitario de Granada (ibs. GRANADA), Granada, Spain. [Martínez,R, López-Jurado,M, Porres,JM] Department of Physiology, Institute of Nutrition and Food Technology (INyTA), Biomedical Research Center (CIBM), Universidad de Granada, Granada, Spain. [Galisteo,M] Department of Pharmacology, School of Pharmacy, University of Granada, Granada, Spain., This research was funded by the Granada University (project PSE/17/002), Ministerio de Ciencia e Innovación (project RTC-2017-6540-1) and by the CTS-107 and AGR145 Groups. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Anatomy::Cells::Cells
Cultured::Cell Line::Cell Line Tumor [Medical Subject Headings] 0301 basic medicine cancer stem cells Etanol Organisms::Eukaryota::Plants::Viridiplantae::Streptophyta::Embryophyta::Angiosperms::Euphorbiaceae::Euphorbia [Medical Subject Headings] Colorectal cancer Apoptosis Pharmacology Antioxidants Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings] Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Molecular Mechanisms of Pharmacological Action::Antioxidants [Medical Subject Headings] 0302 clinical medicine Euphorbia Phenomena and Processes::Circulatory and Respiratory Physiological Phenomena::Cardiovascular Physiological Phenomena::Cardiovascular Physiological Processes::Neovascularization Physiologic [Medical Subject Headings] Antiangiogenic effect Caspase Anatomy::Cells::Epithelial Cells::Endothelial Cells::Human Umbilical Vein Endothelial Cells [Medical Subject Headings] Nutrition and Dietetics biology Chemistry Cancer stem cells apoptosis Colon cancer Anatomy::Plant Structures::Plant Components Aerial::Fruit::Seeds [Medical Subject Headings] colon cancer Euphorbia lathyris 030220 oncology & carcinogenesis Colonic Neoplasms Seeds Chemicals and Drugs::Pharmaceutical Preparations::Plant Extracts [Medical Subject Headings] lcsh:Nutrition. Foods and food supply Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Antineoplastic Agents::Antineoplastic Agents Phytogenic [Medical Subject Headings] Proliferación celular Programmed cell death Polifenoles ethanolic extract Neovascularization Physiologic lcsh:TX341-641 Adenocarcinoma 03 medical and health sciences Cancer stem cell In vivo Cell Line Tumor medicine Human Umbilical Vein Endothelial Cells Humans Neoplasias del colon Cell Proliferation Ethanol antiangiogenic effect Plant Extracts Diseases::Neoplasms::Neoplasms by Histologic Type::Neoplasms Germ Cell and Embryonal::Neuroectodermal Tumors::Neoplasms Neuroepithelial::Glioma::Astrocytoma::Glioblastoma [Medical Subject Headings] Polyphenols medicine.disease biology.organism_classification Antineoplastic Agents Phytogenic Ethanolic extract 030104 developmental biology Polyphenol Diseases::Neoplasms::Neoplasms by Site::Digestive System Neoplasms::Gastrointestinal Neoplasms::Intestinal Neoplasms::Colorectal Neoplasms::Colonic Neoplasms [Medical Subject Headings] biology.protein Glioblastoma Food Science |
| Zdroj: | Digibug. Repositorio Institucional de la Universidad de Granada instname Nutrients Volume 13 Issue 2 Digibug: Repositorio Institucional de la Universidad de Granada Universidad de Granada (UGR) Nutrients, Vol 13, Iss 566, p 566 (2021) |
| Popis: | The seeds of Euphorbia lathyris have been used in traditional medicine to treat various medical conditions. However, neither all of their active biocompounds nor the molecular mechanisms underlying their therapeutic effects have been described. A new ethanolic extract of defatted flour from mature seeds of Euphorbia lathyris showed a high total polyphenol content and significant antioxidant activity. Chromatographic analysis showed that esculetin, euphorbetin, gaultherin, and kaempferol-3-rutinoside were the most abundant polyphenolic bioactive compounds. Antiproliferative assays showed a high and selective antitumor activity against colon cancer cell lines (T84 and HCT-15). In addition, a significant antiproliferative activity against glioblastoma multiforme cells was also demonstrated. Its mechanism of action to induce cell death was mediated by the overexpression of caspases 9, 3, and 8, and by activation of autophagy. Interestingly, a reduction in the migration capacity of colon cancer cells and a significant antiangiogenic effect on human umbilical vein endothelial cells were also demonstrated. Finally, the extract significantly reduced the subpopulations of cancer stem cells. This extract could be the basis to develop new therapeutic strategies for the treatment of colon cancer, although further experiments will be necessary to determine its in vivo effects. Granada University (project PSE/17/002) Ministerio de Ciencia e Innovación (project RTC-2017-6540-1) CTS-107 and AGR145 Groups |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|