Development of a method to cryopreserve Greenshell mussel™ (Perna canaliculus) veliger larvae
| Autor: | Joanna Copedo, Jolene Berry, Serean L. Adams, Pablo Heres, Julien Vignier, Estefania Paredes |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Ethylene Glycol
Perna animal structures Veliger Selective breeding Cryopreservation General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Animal science Animals Perna canaliculus 2401.19 Zoología Marina Larva 030219 obstetrics & reproductive medicine 2407.01 Cultivo Celular biology fungi 0402 animal and dairy science 04 agricultural and veterinary sciences Mussel General Medicine Straw biology.organism_classification 040201 dairy & animal science Hatchery 2510.92 Acuicultura Marina General Agricultural and Biological Sciences New Zealand |
| Zdroj: | Investigo. Repositorio Institucional de la Universidade de Vigo Universidade de Vigo (UVigo) |
| Popis: | Cryopreservation of larvae of Greenshell™ mussel Perna canaliculus, the most cultivated species in New Zealand, can provide flexibility for selective breeding programmes and enhance its global production. In this study, we set out to develop a reliable protocol for freezing D-stage larvae of Greenshell™ mussels that ensured long-term survival for successful rearing of thawed larvae in the hatchery. The effects of different combinations of cryoprotecting agents (CPA), varying CPA equilibration times, larval concentrations per straw as well as different larval development stages (48 h vs 72 h old) were evaluated by assessing the behavioural response (swimming activity, algal consumption), shell size and survival of larvae, up to 4 days post-thawing. The protocol yielding the best larval performances was a combination of the following CPA (final concentrations): 14% ethylene-glycol (EG) + 0.6 M trehalose (TRE) + 1% polyvinyl-pyrrolidone (PVP), prepared with Milli-Q water. Stocking densities ranging from 50,000 to 150,000 larvae per straw (0.25 mL) and a 20 min equilibration time gave the best results, while no significant differences in fitness were found between larvae cryopreserved at 48 h nor 72 h-old. Using the improved cryopreservation protocol, over 50% of previously cryopreserved D-larvae were able to survive after 4 days of rearing, compared with 65% in the unfrozen control. More importantly, about one third of thawed larvae were able to swim and feed, and to potentially develop further. These findings contribute to enhance the selective breeding programmes for this species. Xunta de Galicia Ministry for Business, Innovation and Employment (New Zealand) Shellfish Aquaculture Ref. CAWX1801 |
| Databáze: | OpenAIRE |
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