Miniature flow-injection analysis manifold created by micromilling

Autor:	Andrea Rainelli, Richard Stratz, Karin Schweizer, Peter C. Hauser
Rok vydání:	2003
Předmět:	Flow injection analysis Optical fiber business.industry Chemistry Microfluidics Analytical chemistry Analytical Chemistry law.invention Absorbance Electrokinetic phenomena Optics law Reagent Miniaturization business Manifold (fluid mechanics)
Zdroj:	Talanta. 61(5)
ISSN:	1873-3573
Popis:	The miniature flow injection analysis (μFIA) system is based on a mechanically engraved manifold in a PMMA-substrate. The channels have a trapezoidal cross-section with a depth of 150 μm and a width between 180 and 360 μm and the reactor volume is 3.75 μl. A light-emitting diode (LED) is used as radiation source for absorbance detection and optical fibres are used for transmitting light to and from the manifold. Computer controlled valves are used for directing the liquid flows maintained with syringe pumps. A sampling rate of approximately five determinations per minute was achieved with a reagent consumption of 10 μl min −1 . Four applications were implemented and tested; the performance was found to be comparable to that obtained with conventional FIA-set-ups despite the drastically reduced optical pathlength. Electrokinetic pumping was extensively evaluated but found to generally be very restricted in scope because the reagent solutions cannot usually be adapted to the requirements for generating the flow.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8b16296581f061c1baff65032ac0e3af https://pubmed.ncbi.nlm.nih.gov/18969230 Zobrazit plný text záznamu Full Text from ScienceDirect