The endothelial nitric oxide synthase gene G894T, glutathione S-transferase (GSTM1 and GSTT1) polymorphisms as a risk factor in the patient with nephrolithiasis
| Autor: | Atheer Awad Mehde, Wesen Adel Mehdi, Raha Ahmed Raus, Hamid Ghazali, Faridah Yusof, Ali Khudhair Resen |
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| Rok vydání: | 2019 |
| Předmět: |
Male
medicine.medical_specialty Genotype Nitric Oxide Synthase Type III 02 engineering and technology medicine.disease_cause Nephrolithiasis Biochemistry 03 medical and health sciences chemistry.chemical_compound Structural Biology Enos Risk Factors Internal medicine medicine Humans Genetic Predisposition to Disease Molecular Biology Gene 030304 developmental biology Glutathione Transferase 0303 health sciences Polymorphism Genetic biology business.industry General Medicine Glutathione Middle Aged 021001 nanoscience & nanotechnology biology.organism_classification Restriction enzyme Endocrinology Glutathione S-transferase chemistry Case-Control Studies biology.protein Female Gene polymorphism 0210 nano-technology business Oxidative stress Polymorphism Restriction Fragment Length |
| Zdroj: | International journal of biological macromolecules. 140 |
| ISSN: | 1879-0003 |
| Popis: | Background The genetic features indicate a crucial role in nephrolithiasis. The present study was aimed to investigate the role of Glutathione-S-transferase Mu (GSTM1), Glutathione-S- transferase Theta (GSTT1) and endothelial nitric oxide synthase (eNOs) gene polymorphism in nephrolithiasis. Methods We involved a case-control study in which 480 individuals were divided into 240 healthy control and 240 patients with nephrolithiasis. For each patient and control, we measured biochemical criteria, levels of glutathione S-transferase, eNOs, GSTM1, GSTT1genes and eNOS genes polymorphism by PCR-RFLP. Results GSTM1 and GSTT1 null genotypes are not a risk features for nephrolithiasis. The eNOS frequency GG, GT, and TT genotypes by using Ban II enzyme as restriction enzyme were found to be (48.33, 36.67, and 15.00) %. The eNOS frequency TT, GT, and GG genotypes by using the Ban II enzyme as restriction enzyme were found to be 15.84, 25.83, and 58.33%, respectively. The result showed an increase in serum eNOs levels were in the patient's group comparing to control. Conclusions This work is the first in the literature to study the relation between eNOs genes polymorphisms and nephrolithiasis. The results conclude that TT genotypes in the eNOs genes are associated with an increase the oxidative stress in patients. |
| Databáze: | OpenAIRE |
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