Superior Effect of MD05, Beta-Tricalcium Phosphate Coated With Recombinant Human Growth/Differentiation Factor-5, Compared to Conventional Bone Substitutes in the Rat Calvarial Defect Model
| Autor: | Michael Siedler, Andreas Schütz, Sylke Poehling, Carola Dony, Susanne D. Pippig, Klaus Hellerbrand |
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| Rok vydání: | 2006 |
| Předmět: |
Calcium Phosphates
Male Bone Regeneration Dentistry GDF5 Bone morphogenetic protein Statistics Nonparametric law.invention Rats Sprague-Dawley chemistry.chemical_compound Growth Differentiation Factor 5 Transforming Growth Factor beta law medicine Animals Humans Bone regeneration Calvarial defect business.industry Skull Growth differentiation factor Phosphate Recombinant Proteins Rats Specific Pathogen-Free Organisms medicine.anatomical_structure chemistry Bone Morphogenetic Proteins Bone Substitutes Models Animal Recombinant DNA Periodontics Cattle Female Collagen business Biomedical engineering |
| Zdroj: | Journal of Periodontology. 77:1582-1590 |
| ISSN: | 1943-3670 0022-3492 |
| DOI: | 10.1902/jop.2006.050328 |
| Popis: | MD05 consists of beta-tricalcium phosphate (beta-TCP) coated with recombinant human growth/differentiation factor-5 (rhGDF-5) and is under evaluation as an osteoinductive and osteoconductive bone graft material for use in dental and maxillofacial applications. The objective of this study was to compare the bone regenerative properties of MD05 with those of conventional commercially available bone substitutes.Full-thickness, 6-mm diameter, calvarial critical-size defects (two per animal) were created in adult Sprague-Dawley rats. Groups of rats were implanted with the following: 1) MD05; 2) bovine bone mineral; 3) bovine bone mineral with collagen; 4) bovine bone mineral with synthetic peptide, 5) beta-TCP (from two different manufacturers); or 6) no filling material (sham controls). Blinded macroscopic analysis, histopathologic analysis, and histomorphometric analysis were carried out 6 weeks after implantation.New bone formation assessed histomorphometrically was about five times greater with MD05 than with the other bone substitutes tested, and bone repair was well advanced in MD05-filled defects after 6 weeks. The extent of fibrous tissue and residual implant were significantly lower in the MD05 group. In contrast to the other materials, the use of MD05 was associated with the complete osseous bridging of the defect and with the presence of normal bone marrow. The osteoinductive effect of rhGDF-5 was apparent from the more pronounced bone ingrowth observed with MD05 compared to the beta-TCP carrier alone. All implants showed good biocompatibility.MD05 achieved superior bone regeneration compared to conventional materials and is a promising new bone substitute for dental and maxillofacial applications. |
| Databáze: | OpenAIRE |
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