A Dominant Negative RAF-1 Mutant Prevents V-SRC-Induced Transformation
| Autor: | M. Hendrickson, Jianguo Song, David A. Foster, U. Rapp, J. Bruder, S. A. Qureshi, Ruchika Gupta, C. K. Joseph |
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| Rok vydání: | 1993 |
| Předmět: |
Mutant
Biophysics Reversion Protein Serine-Threonine Kinases Biology Biochemistry 3T3 cells Oncogene Protein pp60(v-src) Diglycerides Gene product Mice Proto-Oncogene Proteins medicine Animals Phosphotyrosine Molecular Biology Cells Cultured Genes Dominant Mice Inbred BALB C 3T3 Cells Cell Biology Transfection Molecular biology Proto-Oncogene Proteins c-raf Transformation (genetics) Cell Transformation Neoplastic medicine.anatomical_structure Mutation v-Src Tyrosine Intracellular |
| Zdroj: | Biochemical and Biophysical Research Communications. 192:969-975 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1993.1510 |
| Popis: | A vector expressing a dominant negative mutant of Raf-1 was stably introduced into BALB/c 3T3 cells expressing a temperature-sensitive derivative of v-Src. High levels of the Raf-mutant were detected in these cells. Expression of the Raf-1 mutant blocked v-Src-induced transformation, as determined by reversion to a flat non-transformed morphology and the inability to form colonies in soft agar. Cells transfected with the parental vector lacking the mutant Raf-1 could be transformed by v-Src. These data suggest that intracellular signals activated by v-Src that are mediated by Raf-1 are required for transformation by v-Src. |
| Databáze: | OpenAIRE |
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