Enhanced Fibrinolysis with Magnetically Powered Colloidal Microwheels
| Autor: | Rogier M. Schoeman, Tonguc O. Tasci, Keith B. Neeves, Dante Disharoon, Paco S. Herson, David W. M. Marr, Kuldeepsinh Rana |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Materials science
Time Factors medicine.medical_treatment Nanotechnology 02 engineering and technology 030204 cardiovascular system & hematology Fibrin Article Biomaterials 03 medical and health sciences 0302 clinical medicine Fibrinolysis medicine Humans General Materials Science Platelet Colloids Thrombus biology Biological Transport Thrombosis General Chemistry Penetration (firestop) 021001 nanoscience & nanotechnology medicine.disease Magnetic Fields Pharmaceutical Preparations Hemostasis Tissue Plasminogen Activator biology.protein 0210 nano-technology Plasminogen activator Fibrinolytic agent Biotechnology Biomedical engineering |
| Zdroj: | Small |
| ISSN: | 1613-6829 |
| Popis: | Thrombi that occlude blood vessels can be resolved with fibrinolytic agents that degrade fibrin, the polymer that forms between and around platelets to provide mechanical stability. Fibrinolysis rates however are often constrained by transport-limited delivery to and penetration of fibrinolytics into the thrombus. Here, these limitations are overcome with colloidal microwheel (μwheel) assemblies functionalized with the fibrinolytic tissue-type plasminogen activator (tPA) that assemble, rotate, translate, and eventually disassemble via applied magnetic fields. These microwheels lead to rapid fibrinolysis by delivering a high local concentration of tPA to induce surface lysis and, by taking advantage of corkscrew motion, mechanically penetrating into fibrin gels and platelet-rich thrombi to initiate bulk degradation. Fibrinolysis of plasma-derived fibrin gels by tPA-microwheels is fivefold faster than with 1 μg mL(−1) tPA. μWheels following corkscrew trajectories can also penetrate through 100 μm sized platelet-rich thrombi formed in a microfluidic model of hemostasis in ≈5 min. This unique combination of surface and bulk dissolution mechanisms with mechanical action yields a targeted fibrinolysis strategy that could be significantly faster than approaches relying on diffusion alone, making it well-suited for occlusions in small or penetrating vessels not accessible to catheter-based removal. |
| Databáze: | OpenAIRE |
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