Purification and initial characterisation of koala immunoglobulins
| Autor: | M. Allanson, D. Lawrence, R. Wilkinson, S. Neville, V. Kolega |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Male
Immunodiffusion Immunology Serum albumin Immunoelectrophoresis Chromatography Affinity Affinity chromatography Phascolarctos cinereus biology.animal medicine Animals Bovine serum albumin Electrophoresis Agar Gel Gel electrophoresis General Veterinary biology medicine.diagnostic_test Molecular biology Marsupialia Immunoglobulin G biology.protein Electrophoresis Polyacrylamide Gel Rabbits Protein G Antibody |
| Zdroj: | Veterinary Immunology and Immunopathology. 29:189-195 |
| ISSN: | 0165-2427 |
| DOI: | 10.1016/0165-2427(91)90064-j |
| Popis: | The production of koala immunoglobulins (Ig) was elicited by the immunisation of a koala ( Phascolarctos cinereus ) with bovine serum albumin. This Ig was then purified using the highly specific techniques of affinity chromatography. The purified protein was compared with a “potential” koala Ig protein which was subsequently purified by Protein G chromatography and both proteins were further characterised by agarose/SDS-PAGE and immunoelectrophoresis. Results indicate that koalas do produce Ig in response to antigen challenge, koala Ig has a higher net negative charge than that seen in most other mammals and possibly two subclasses of IgG and IgM are present in normal koala serum. |
| Databáze: | OpenAIRE |
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