Rotavirus strain distribution in Ghana pre- and post- rotavirus vaccine introduction
| Autor: | Jason M. Mwenda, Emmanuel Addo-Yobo, Daniel Ansong, Belinda Lartey, Sandra Kwarteng-Owusu, Susan Damanka, George Armah, Christabel Enweronu-Laryea, Fred Binka, Clement T. Narh, Ben Lopman, Stanley K. Diamenu, Francis E. Dennis, Kwamena W. Sagoe, Umesh D. Parashar |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Rotavirus
0301 basic medicine Vaccination Coverage Genotype viruses 030106 microbiology Biology Vaccines Attenuated medicine.disease_cause Ghana Rotavirus Infections West africa Immunoenzyme Techniques Feces 03 medical and health sciences Prevalence medicine Humans Antigens Viral Pre and post Phylogeny General Veterinary General Immunology and Microbiology Immunization Programs Rotavirus Vaccines Public Health Environmental and Occupational Health Infant virus diseases Sequence Analysis DNA Rotavirus vaccine Virology Vaccine introduction Gastroenteritis Vaccination 030104 developmental biology Infectious Diseases Strain distribution Child Preschool RNA Viral Molecular Medicine Capsid Proteins |
| Zdroj: | Vaccine. 36:7238-7242 |
| ISSN: | 0264-410X |
| Popis: | Ghana introduced the monovalent rotavirus vaccine (Rotarix) into its national paediatric vaccination programme in May2012. Vaccine introduction was initiated nationwide and achieved85% coverage within a few months. Rotavirus strain distribution pre- and post-RV vaccine introduction is reported.Stool samples were collected from diarrhoeic children5 years of age hospitalized between 2009 and 2016 at sentinel sites across Ghana and analyzed for the presence of group A rotavirus by enzyme immunoassay. Rotavirus strains were characterized by RT-PCR and sequencing.A total of 1363 rotavirus EIA-positive samples were subjected to molecular characterization. These were made up of 823 (60.4%) and 540 (39.6%) samples from the pre- and post-vaccine periods respectively. Rotavirus VP7 genotypes G1, G2 and G3, and VP4 genotypes P[6] and P[8] constituted more than 65% of circulating G and P types in the pre-vaccine period. The common strains detected were G1P[8] (20%), G3P[6] (9.2%) and G2P[6] (4.9%). During the post-vaccine period, G12, G1 and G10 genotypes, constituted more than 65% of the VP7 genotypes whilst P[6] and P[8] made up more than 75% of the VP4 genotypes. The predominant circulating strains were G12P[8] (26%), G10P[6] (10%) G3P[6] (8.1%) and G1P[8] (8.0%). We also observed the emergence of the unusual rotavirus strain G9P[4] during this period.Rotavirus G1P[8], the major strain in circulation during the pre-vaccination era, was replaced by G12P[8] as the most predominant strain after vaccine introduction. This strain replacement could be temporary and unrelated to vaccine introduction since an increase in G12 was observed in countries yet to introduce the rotavirus vaccine in West Africa. A continuous surveillance programme in the post-vaccine era is necessary for the monitoring of circulating rotavirus strains and the detection of unusual/emerging genotypes. |
| Databáze: | OpenAIRE |
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