Adult Zebrafish (Danio rerio): An Alternative Behavioral Model of Formalin-Induced Nociception
| Autor: | Francisco Ernani Alves Magalhães, Adriana Rolim Campos, Sacha Aubrey Alves Rodrigues Santos, Ramon da Silva Raposo, Messias Vital de Oliveira, Renata Barbosa Menezes, Caio Átila Prata Bezerra de Sousa, Francisco Lucas Alves Batista, Luiz Francisco Wemmenson Gonçalves Moura, Ângela Oliveira Abreu |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Nociception endocrine system animal structures animal diseases Indomethacin Danio 03 medical and health sciences 0302 clinical medicine stomatognathic system Formaldehyde Animals Laboratory research Zebrafish biology Behavior Animal Morphine fungi Anti-Inflammatory Agents Non-Steroidal Anatomy biology.organism_classification Analgesics Opioid Disease Models Animal 030104 developmental biology Animal Science and Zoology Neuroscience 030217 neurology & neurosurgery Locomotion Developmental Biology |
| Zdroj: | Zebrafish. 14(5) |
| ISSN: | 1557-8542 |
| Popis: | The zebrafish (Danio rerio) has been proposed as a low-cost and simple alternative to the use of higher vertebrates in laboratory research on novel compounds with antinociceptive potential. In this study, we tested adult zebrafish (Danio rerio) as an alternative behavioral model of formalin-induced nociception. We evaluated the nociceptive effect of 0.1% formalin (3 or 5 μL; intramuscularly [i.m.]), applied into the tail or lips, on locomotor activity, using as parameter the number of times the fish crossed the lines between the quadrants of a glass Petri dish during the neurogenic stage (0-5 min) and the inflammatory stage (15-30 min). The behavioral model was validated by testing the antinociceptive effect of morphine and indomethacin (standard analgesic drugs used in the formalin test of rodents). We also tested whether the effect of morphine could be modulated by naloxone, an opioid antagonist. The effect of morphine and indomethacin on zebrafish locomotor behavior was evaluated with the open field test. The white/black test was used to rule out the anxiolytic effect of 0.1% formalin injected into the tail on adult zebrafish. Formalin (0.1%; 3 and 5 μL injected into the tail) increased significantly the nociceptive behavior of the adult zebrafish in both stages (p 0.001 vs. control). Morphine and indomethacin (both 0.2 mg/mL; 20 μL; intraperitoneally [i.p.]) significantly inhibited nociception induced with formalin (5 μL injected i.m. into the tail) in both stages (p 0.001). Naloxone blocked the antinociceptive effect of morphine. No influence on locomotion was observed. Locally administered formalin (injected into the tail) induced nociception, but not anxiety. The results suggest that the adult zebrafish behavioral model is a feasible alternative to more conventional laboratory models used in research on novel compounds with antinociceptive potential. |
| Databáze: | OpenAIRE |
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