DNA ploidy and p53 expression correlate with survival and cell proliferative activity in male breast carcinoma
| Autor: | E Margaria, Massimo Geuna, Luigi Chiusa, Achille Pich, Renata Ponti |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Time Factors p53 expression Mammary gland Gene Expression Estrogen receptor male breast carcinoma Breast Neoplasms Male Pathology and Forensic Medicine DNA ploidy proliferative activity prognosis Andrology Progesterone receptor medicine Carcinoma Humans Male Breast Carcinoma Aged Aged 80 and over Ploidies biology DNA Neoplasm Middle Aged Flow Cytometry Prognosis medicine.disease Immunohistochemistry Survival Analysis Proliferating cell nuclear antigen medicine.anatomical_structure biology.protein Tumor Suppressor Protein p53 Nucleolus organizer region Cell Division |
| Zdroj: | Human Pathology. 27:676-682 |
| ISSN: | 0046-8177 |
| DOI: | 10.1016/s0046-8177(96)90397-4 |
| Popis: | DNA flow cytometry and the monoclonal antibody D07 were applied in formalin-fixed, paraffin-embedded specimens from 34 primary male breast carcinomas to verify whether DNA ploidy and p53 expression were associated with survival and proliferative activity. They were compared with tumor clinicopathologic features, sex steroid hormone receptors and cell proliferative activity, assessed by the counts of the argyrophilic nucleolar organizer regions (AgNORs), the monoclonal antibody PC10 against the proliferating cell nuclear antigen and the monoclonal antibody MIB-1. A significant correlation was found between survival and tumor ploidy (median survival, 77 months for diploid but only 38 months for aneuploid cases; P = .03) and p53 expression (median survival, 95 months for cases with p53 scores ⩽ 14.06% versus 33 for cases with p53 scores > 14.06%; P = .0004; median survival, 99 months for p53 negative vs 39 for positive cases; P = .007). Tumor histological grade ( P = .006), AgNOR counts ( P = .0001), PC10 scores ( P = .002), and MIB-1 scores ( P = .001) were also associated with prognosis. In the multivariate analysis, only p53 scores ( P = .001) or p53 immunopositivity ( P = .003) and AgNOR counts ( P = .022) retained an independent prognostic significance. Aneuploid tumors had higher AgNOR counts ( P = .002), PC10 ( P = .007), MIB-1 ( P = .006), and p53 scores ( P = .01) than diploid cases. A linear relationship was observed between p53 scores and AgNOR counts ( r = .41; P = .014), PC10 ( r = .46; P = .005), and MIB-1 scores ( r = .44; P = .011). These results indicate that DNA ploidy and p53 expression are associated with survival and cell proliferative activity in male breast carcinoma. Quantitative parameters, such as DNA ploidy, p53 scores, AgNOR counts, PC10, and MIB-1 scores substantially improve the prognostic significance of the traditional parameters in male breast carcinoma. |
| Databáze: | OpenAIRE |
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